5zdr

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==Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with ascofuranone derivative==
==Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with ascofuranone derivative==
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<StructureSection load='5zdr' size='340' side='right' caption='[[5zdr]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
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<StructureSection load='5zdr' size='340' side='right'caption='[[5zdr]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5zdr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZDR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZDR FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5zdr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZDR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZDR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHW:3-CHLORO-4,6-DIHYDROXY-5-[(2E,6E,8S)-8-HYDROXY-3,7-DIMETHYLNONA-2,6-DIEN-1-YL]-2-METHYLBENZALDEHYDE'>CHW</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHW:3-CHLORO-4,6-DIHYDROXY-5-[(2E,6E,8S)-8-HYDROXY-3,7-DIMETHYLNONA-2,6-DIEN-1-YL]-2-METHYLBENZALDEHYDE'>CHW</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vva|3vva]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vva|3vva]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AOX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 TRYBB])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zdr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zdr OCA], [http://pdbe.org/5zdr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zdr RCSB], [http://www.ebi.ac.uk/pdbsum/5zdr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zdr ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zdr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zdr OCA], [http://pdbe.org/5zdr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zdr RCSB], [http://www.ebi.ac.uk/pdbsum/5zdr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zdr ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/AOX_TRYBB AOX_TRYBB]] Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.
[[http://www.uniprot.org/uniprot/AOX_TRYBB AOX_TRYBB]] Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The alternative oxidase (AOX) is a monotopic diiron carboxylate protein which catalyzes the four-electron reduction of dioxygen to water by ubiquinol. Although we have recently determined the crystal structure of Trypanosoma brucei AOX (TAO) in the presence and absence of ascofuranone (AF) derivatives (which are potent mixed type inhibitors) the mechanism by which ubiquinol and dioxygen binds to TAO remain inconclusive. In this article, ferulenol was identified as the first competitive inhibitor of AOX which has been used to probe the binding of ubiquinol. Surface plasmon resonance reveals that AF is a quasi-irreversible inhibitor of TAO whilst ferulenol binding is completely reversible. The structure of the TAO-ferulenol complex, determined at 2.7A, provided insights into ubiquinol binding and has also identified a potential dioxygen molecule bound in a side-on conformation to the diiron center for the first time.
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Insights into the ubiquinol/dioxygen binding and proton relay pathways of the alternative oxidase.,Shiba T, Inaoka DK, Takahashi G, Tsuge C, Kido Y, Young L, Ueda S, Balogun EO, Nara T, Honma T, Tanaka A, Inoue M, Saimoto H, Harada S, Moore AL, Kita K Biochim Biophys Acta Bioenerg. 2019 Mar 22;1860(5):375-382. doi:, 10.1016/j.bbabio.2019.03.008. PMID:30910528<ref>PMID:30910528</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5zdr" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
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[[Category: Trybb]]
[[Category: Balogun, E O]]
[[Category: Balogun, E O]]
[[Category: Harada, S]]
[[Category: Harada, S]]

Revision as of 08:06, 3 April 2019

Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with ascofuranone derivative

PDB ID 5zdr

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