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6dgb
From Proteopedia
(Difference between revisions)
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==Crystal structure of the C-terminal catalytic domain of IS1535 TnpA, an IS607-like serine recombinase== | ==Crystal structure of the C-terminal catalytic domain of IS1535 TnpA, an IS607-like serine recombinase== | ||
| - | <StructureSection load='6dgb' size='340' side='right' caption='[[6dgb]], [[Resolution|resolution]] 2.52Å' scene=''> | + | <StructureSection load='6dgb' size='340' side='right'caption='[[6dgb]], [[Resolution|resolution]] 2.52Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6dgb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dgb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgb OCA], [http://pdbe.org/6dgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgb RCSB], [http://www.ebi.ac.uk/pdbsum/6dgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgb ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IS1535 TnpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgb OCA], [http://pdbe.org/6dgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgb RCSB], [http://www.ebi.ac.uk/pdbsum/6dgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | IS607-family transposons are unusual because they do not have terminal inverted repeats or generate target site duplications. They encode two protein-coding genes, but only tnpA is required for transposition. Our X-ray structures confirm that TnpA is a member of the serine recombinase (SR) family, but the chemically-inactive quaternary structure of the dimer, along with the N-terminal location of the DNA binding domain, are different from other SRs. TnpA dimers from IS1535 cooperatively associate with multiple subterminal repeats, which together with additional nonspecific binding, form a nucleoprotein filament on one transposon end that efficiently captures a second unbound end to generate the paired-end complex (PEC). Formation of the PEC does not require a change in the dimeric structure of the catalytic domain, but remodeling of the C-terminal alpha-helical region is involved. We posit that the PEC recruits a chemically-active conformer of TnpA to the transposon end to initiate DNA chemistry. | ||
| + | |||
| + | Multiple serine transposase dimers assemble the transposon-end synaptic complex during IS607-family transposition.,Chen W, Mandali S, Hancock SP, Kumar P, Collazo M, Cascio D, Johnson RC Elife. 2018 Oct 5;7. pii: 39611. doi: 10.7554/eLife.39611. PMID:30289389<ref>PMID:30289389</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6dgb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Cascio, D]] | [[Category: Cascio, D]] | ||
[[Category: Chen, W Y]] | [[Category: Chen, W Y]] | ||
Revision as of 08:10, 3 April 2019
Crystal structure of the C-terminal catalytic domain of IS1535 TnpA, an IS607-like serine recombinase
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