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6ehm

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Revision as of 08:12, 3 April 2019

Model of the Ebola virus nucleocapsid subunit from recombinant virus-like particles

Structural highlights

6ehm is a 4 chain structure with sequence from Ebov-may. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	NP (EBOV-May), VP24 (EBOV-May)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NCAP_EBOZM] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases. [VP24_EBOZM] Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. Blocks the IFN-induced nuclear accumulation of host phosphorylated STAT1, by interacting with the STAT1-binding region of host importin alpha-1/KPNA1 protein, thereby inhibiting the latter. Without the activity of this protein, activated STAT1 would not enter the nucleus and be unable to activate IFN-induced genes. Plays a role in assembly of viral nucleocapsid and virion budding. May act as a minor matrix protein that plays a role in assembly of viral nucleocapsid and virion budding.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles virus, and respiratory syncytial virus. Mononegaviruses have non-segmented, single-stranded negative-sense RNA genomes that are encapsidated by nucleoprotein and other viral proteins to form a helical nucleocapsid. The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcription and replication. Insights into nucleoprotein-nucleoprotein interactions have been derived from structural studies of oligomerized, RNA-encapsidating nucleoprotein, and cryo-electron microscopy of nucleocapsid or nucleocapsid-like structures. There have been no high-resolution reconstructions of complete mononegavirus nucleocapsids. Here we apply cryo-electron tomography and subtomogram averaging to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies. These structures reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation of an extended alpha-helix from the disordered carboxy-terminal region of nucleoprotein-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and accessory protein recruitment.

Structure and assembly of the Ebola virus nucleocapsid.,Wan W, Kolesnikova L, Clarke M, Koehler A, Noda T, Becker S, Briggs JAG Nature. 2017 Nov 16;551(7680):394-397. doi: 10.1038/nature24490. Epub 2017 Nov 8. PMID:29144446^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huang Y, Xu L, Sun Y, Nabel GJ. The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol Cell. 2002 Aug;10(2):307-16. PMID:12191476
↑ Han Z, Boshra H, Sunyer JO, Zwiers SH, Paragas J, Harty RN. Biochemical and functional characterization of the Ebola virus VP24 protein: implications for a role in virus assembly and budding. J Virol. 2003 Feb;77(3):1793-800. PMID:12525613
↑ Licata JM, Johnson RF, Han Z, Harty RN. Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles. J Virol. 2004 Jul;78(14):7344-51. PMID:15220407 doi:http://dx.doi.org/10.1128/JVI.78.14.7344-7351.2004
↑ Reid SP, Valmas C, Martinez O, Sanchez FM, Basler CF. Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1. J Virol. 2007 Dec;81(24):13469-77. Epub 2007 Oct 10. PMID:17928350 doi:http://dx.doi.org/10.1128/JVI.01097-07
↑ Wan W, Kolesnikova L, Clarke M, Koehler A, Noda T, Becker S, Briggs JAG. Structure and assembly of the Ebola virus nucleocapsid. Nature. 2017 Nov 16;551(7680):394-397. doi: 10.1038/nature24490. Epub 2017 Nov 8. PMID:29144446 doi:http://dx.doi.org/10.1038/nature24490

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ehm"

@@ Line 1: / Line 1: @@
 ==Model of the Ebola virus nucleocapsid subunit from recombinant virus-like particles==
-<StructureSection load='6ehm' size='340' side='right' caption='[[6ehm]], [[Resolution|resolution]] 7.30&Aring;' scene=''>
+<StructureSection load='6ehm' size='340' side='right'caption='[[6ehm]], [[Resolution|resolution]] 7.30&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6ehm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebov-may Ebov-may]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EHM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EHM FirstGlance]. <br>
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 6ehm" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Nucleoprotein|Nucleoprotein]]
+*[[VP24|VP24]]
 == References ==
 <references/>
@@ Line 23: / Line 27: @@
 </StructureSection>
 [[Category: Ebov-may]]
+[[Category: Large Structures]]
 [[Category: Becker, S]]
 [[Category: Briggs, J A.G]]

6ehm

From Proteopedia

Revision as of 08:12, 3 April 2019

Model of the Ebola virus nucleocapsid subunit from recombinant virus-like particles

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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