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4jgz

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Revision as of 08:54, 3 April 2019

Crystal structure of human coxsackievirus A16 uncoating intermediate (space group I222)

Structural highlights

4jgz is a 3 chain structure with sequence from Human coxsackievirus a16. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[I3W9E1_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

Publication Abstract from PubMed

It remains largely mysterious how the genomes of non-enveloped eukaryotic viruses are transferred across a membrane into the host cell. Picornaviruses are simple models for such viruses, and initiate this uncoating process through particle expansion, which reveals channels through which internal capsid proteins and the viral genome presumably exit the particle, although this has not been clearly seen until now. Here we present the atomic structure of an uncoating intermediate for the major human picornavirus pathogen CAV16, which reveals VP1 partly extruded from the capsid, poised to embed in the host membrane. Together with previous low-resolution results, we are able to propose a detailed hypothesis for the ordered egress of the internal proteins, using two distinct sets of channels through the capsid, and suggest a structural link to the condensed RNA within the particle, which may be involved in triggering RNA release.

Picornavirus uncoating intermediate captured in atomic detail.,Ren J, Wang X, Hu Z, Gao Q, Sun Y, Li X, Porta C, Walter TS, Gilbert RJ, Zhao Y, Axford D, Williams M, McAuley K, Rowlands DJ, Yin W, Wang J, Stuart DI, Rao Z, Fry EE Nat Commun. 2013 Jun 3;4:1929. doi: 10.1038/ncomms2889. PMID:23728514^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ren J, Wang X, Hu Z, Gao Q, Sun Y, Li X, Porta C, Walter TS, Gilbert RJ, Zhao Y, Axford D, Williams M, McAuley K, Rowlands DJ, Yin W, Wang J, Stuart DI, Rao Z, Fry EE. Picornavirus uncoating intermediate captured in atomic detail. Nat Commun. 2013 Jun 3;4:1929. doi: 10.1038/ncomms2889. PMID:23728514 doi:10.1038/ncomms2889

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4jgz"

@@ Line 1: / Line 1: @@
 ==Crystal structure of human coxsackievirus A16 uncoating intermediate (space group I222)==
-<StructureSection load='4jgz' size='340' side='right' caption='[[4jgz]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+<StructureSection load='4jgz' size='340' side='right'caption='[[4jgz]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4jgz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cv-a16 Cv-a16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JGZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JGZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4jgz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_coxsackievirus_a16 Human coxsackievirus a16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JGZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JGZ FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jgz OCA], [http://pdbe.org/4jgz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jgz RCSB], [http://www.ebi.ac.uk/pdbsum/4jgz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jgz ProSAT]</span></td></tr>
 </table>
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Cv-a16]]
+[[Category: Human coxsackievirus a16]]
+[[Category: Large Structures]]
 [[Category: Axford, D]]
 [[Category: Fry, E E]]

4jgz

From Proteopedia

Revision as of 08:54, 3 April 2019

Crystal structure of human coxsackievirus A16 uncoating intermediate (space group I222)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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