5yep
From Proteopedia
(Difference between revisions)
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==Crystal structure of SO_3166-SO_3165 from Shewanella oneidensis== | ==Crystal structure of SO_3166-SO_3165 from Shewanella oneidensis== | ||
| - | <StructureSection load='5yep' size='340' side='right' caption='[[5yep]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='5yep' size='340' side='right'caption='[[5yep]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5yep]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YEP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YEP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yep]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sheon Sheon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YEP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YEP FirstGlance]. <br> |
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SO_3165 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=211586 SHEON]), SO_3166 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=211586 SHEON])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yep OCA], [http://pdbe.org/5yep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yep RCSB], [http://www.ebi.ac.uk/pdbsum/5yep PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yep ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yep OCA], [http://pdbe.org/5yep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yep RCSB], [http://www.ebi.ac.uk/pdbsum/5yep PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yep ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Toxin-antitoxin (TA) loci in bacteria are small genetic modules that regulate various cellular activities, including cell growth and death. The two-gene module encoding a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain and a cognate MNT (minimal nucleotidyltransferase) domain have been predicted to represent a novel type II TA system prevalent in archaea and bacteria. However, the neutralization mechanism and cellular targets of the TA family remain unclear. The toxin SO_3166 having a HEPN domain and its cognate antitoxin SO_3165 with an MNT domain constitute a typical type II TA system that regulates cell motility and confers plasmid stability in the bacterium Shewanella oneidensis Here, we report the crystal structure and solution conformation of the SO_3166-SO_3165 pair, representing the first complex structures in this TA family. The structures revealed that SO_3165 and SO_3166 form a tight heterooctamer (at a 2:6 ratio), an organization that is very rare in other TA systems. We also observed that SO_3166 dimerization enables the formation of a deep cleft at the HEPN-domain interface harboring a composite RX4-6H active site that functions as an RNA-cleaving RNase. SO_3165 bound SO_3166 mainly through its two alpha-helices (alpha2 and alpha4), functioning as molecular recognition elements. Moreover, their insertion into the SO_3166 cleft sterically blocked the RX4-6H site or narrowed the cleft to inhibit RNA substrate binding. Structure-based mutagenesis confirmed the important roles of these alpha-helices in SO_3166 binding and inhibition. Our structure-function analysis provides first insights into the neutralization mechanism of the HEPN-MNT TA family. | ||
| + | |||
| + | Structure-function analyses reveal the molecular architecture and neutralization mechanism of a bacterial HEPN-MNT toxin-antitoxin system.,Jia X, Yao J, Gao Z, Liu G, Dong YH, Wang X, Zhang H J Biol Chem. 2018 May 4;293(18):6812-6823. doi: 10.1074/jbc.RA118.002421. Epub, 2018 Mar 19. PMID:29555683<ref>PMID:29555683</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5yep" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sheon]] | ||
[[Category: Dong, Y H]] | [[Category: Dong, Y H]] | ||
[[Category: Gao, Z Q]] | [[Category: Gao, Z Q]] | ||
Revision as of 07:41, 10 April 2019
Crystal structure of SO_3166-SO_3165 from Shewanella oneidensis
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