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6iuv

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Revision as of 07:52, 10 April 2019

Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 3C11

Structural highlights

6iuv is a 6 chain structure with sequence from Human and Influenza a virus (a/hong kong/482/97 (h5n1)). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	H5, HA (Influenza A virus (A/Hong Kong/482/97 (H5N1)))
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q77XR4_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[HAMAP-Rule:MF_04072][SAAS:SAAS01039073] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324]

Publication Abstract from PubMed

Most neutralizing antibodies against highly pathogenic avian influenza A virus H5N1 recognize the receptor-binding site (RBS) on the globular head domain and the stem of H5N1 hemagglutinin (HA). Through comprehensive analysis of multiple human protective antibodies, we previously identified four vulnerable sites (VS1-VS4) on the globular head domain. Among them, the VS1, occupying the opposite side of the RBS on the same HA, was defined by the epitope of antibody 65C6. In this study, we report the crystal structures of two additional human H5N1 antibodies isolated from H5N1-infected individuals, 3C11 and AVFluIgG01, bound to the head at 2.33 and 2.30 A resolution, respectively. These two new antibody epitopes have large overlap with and extend beyond the original VS1. Site-directed mutagenesis experiments identified eight pivotal residues (Ser-126b, Lys-165, Arg-166, Ser-167, Tyr-168, Asn-169, Thr-171, and Asn-172) critical for 65C6, 3C11 and AVFluIgG01 binding and neutralization activities. These residues formed a unique "Y"-shape surface on H5N1 globular head and are highly conserved among H5N1 viruses. Our results further support the existence of a vulnerable site distinct from the RBS and the stem region of H5N1 HA, and future design of immunogens should take this particular site into consideration.

Structural and functional definition of a vulnerable site on the hemagglutinin of highly pathogenic avian influenza A virus H5N1.,Wang P, Zuo Y, Sun J, Zuo T, Zhang S, Guo S, Shi X, Liang M, Zhou P, Zhang L, Wang X J Biol Chem. 2019 Feb 8. pii: RA118.007008. doi: 10.1074/jbc.RA118.007008. PMID:30737282^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang P, Zuo Y, Sun J, Zuo T, Zhang S, Guo S, Shi X, Liang M, Zhou P, Zhang L, Wang X. Structural and functional definition of a vulnerable site on the hemagglutinin of highly pathogenic avian influenza A virus H5N1. J Biol Chem. 2019 Feb 8. pii: RA118.007008. doi: 10.1074/jbc.RA118.007008. PMID:30737282 doi:http://dx.doi.org/10.1074/jbc.RA118.007008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6iuv"

@@ Line 1: / Line 1: @@
 ==Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 3C11==
-<StructureSection load='6iuv' size='340' side='right' caption='[[6iuv]], [[Resolution|resolution]] 2.33&Aring;' scene=''>
+<StructureSection load='6iuv' size='340' side='right'caption='[[6iuv]], [[Resolution|resolution]] 2.33&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6iuv]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/hong_kong/482/97_(h5n1)) Influenza a virus (a/hong kong/482/97 (h5n1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IUV FirstGlance]. <br>
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Sun, J]]
 [[Category: Wang, P]]

6iuv

From Proteopedia

Revision as of 07:52, 10 April 2019

Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 3C11

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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