4nwo
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T33-15== | ==Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T33-15== | ||
| - | <StructureSection load='4nwo' size='340' side='right' caption='[[4nwo]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='4nwo' size='340' side='right'caption='[[4nwo]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4nwo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NWO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4nwo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971] and [http://en.wikipedia.org/wiki/Atcc_700550 Atcc 700550]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NWO FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nwn|4nwn]], [[4nwp|4nwp]], [[4nwq|4nwq]], [[4nwr|4nwr]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nwn|4nwn]], [[4nwp|4nwp]], [[4nwq|4nwq]], [[4nwr|4nwr]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mogA, SO_0065 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70863 ATCC 700550]), aroG, aroH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 "Flavobacterium thermophilum" Yoshida and Oshima 1971])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nwo OCA], [http://pdbe.org/4nwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nwo RCSB], [http://www.ebi.ac.uk/pdbsum/4nwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nwo ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nwo OCA], [http://pdbe.org/4nwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nwo RCSB], [http://www.ebi.ac.uk/pdbsum/4nwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nwo ProSAT]</span></td></tr> | ||
| Line 11: | Line 12: | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/AROH_THETH AROH_THETH]] Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.<ref>PMID:14727008</ref> | [[http://www.uniprot.org/uniprot/AROH_THETH AROH_THETH]] Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.<ref>PMID:14727008</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The self-assembly of proteins into highly ordered nanoscale architectures is a hallmark of biological systems. The sophisticated functions of these molecular machines have inspired the development of methods to engineer self-assembling protein nanostructures; however, the design of multi-component protein nanomaterials with high accuracy remains an outstanding challenge. Here we report a computational method for designing protein nanomaterials in which multiple copies of two distinct subunits co-assemble into a specific architecture. We use the method to design five 24-subunit cage-like protein nanomaterials in two distinct symmetric architectures and experimentally demonstrate that their structures are in close agreement with the computational design models. The accuracy of the method and the number and variety of two-component materials that it makes accessible suggest a route to the construction of functional protein nanomaterials tailored to specific applications. | ||
| + | |||
| + | Accurate design of co-assembling multi-component protein nanomaterials.,King NP, Bale JB, Sheffler W, McNamara DE, Gonen S, Gonen T, Yeates TO, Baker D Nature. 2014 Jun 5;510(7503):103-8. doi: 10.1038/nature13404. Epub 2014 May 25. PMID:24870237<ref>PMID:24870237</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4nwo" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Flavobacterium thermophilum yoshida and oshima 1971]] | ||
| + | [[Category: Atcc 700550]] | ||
[[Category: Chorismate mutase]] | [[Category: Chorismate mutase]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Baker, D]] | [[Category: Baker, D]] | ||
[[Category: Bale, J B]] | [[Category: Bale, J B]] | ||
Revision as of 09:06, 10 April 2019
Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T33-15
| |||||||||||
Categories: Flavobacterium thermophilum yoshida and oshima 1971 | Atcc 700550 | Chorismate mutase | Large Structures | Baker, D | Bale, J B | King, N P | McNamara, D E | Sheffler, W | Yeates, T O | Computational design | Designed protein cage | Isomerase | Mog | Molybdenum cofactor biosynthesis protein | Multimerization | Nanomaterial | Nanostructure | Protein binding | Protein engineering | Self-assembling | Tetrahedron | Two-component
