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6j0b

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Revision as of 06:58, 17 April 2019

Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath-tube complex in extended state

Structural highlights

6j0b is a 24 chain structure with sequence from Phoaa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	PAU_03352, PA-RVA20-21-0170 (PHOAA), PAU_03353, PA-RVA20-21-0171 (PHOAA)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Contractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of extracellular CISs that are present in both bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage tail, containing a hexagonal baseplate complex with six fibers and a capped 117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence of three variants for both tube and sheath proteins, indicating a functional specialization of them during evolution. The terminal hexameric cap docks onto the topmost layer of the inner tube and locks the outer sheath in pre-contraction state with six stretching arms. Our results on the PVC provide a framework for understanding the general mechanism of widespread CISs and pave the way for using them as delivery tools in biological or therapeutic applications.

Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System.,Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019, Mar 21. PMID:30905475^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019, Mar 21. PMID:30905475 doi:http://dx.doi.org/10.1016/j.cell.2019.02.020

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6j0b"

@@ Line 3: / Line 3: @@
 <StructureSection load='6j0b' size='340' side='right'caption='[[6j0b]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6j0b]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J0B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J0B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6j0b]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Phoaa Phoaa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J0B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J0B FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j0b OCA], [http://pdbe.org/6j0b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j0b RCSB], [http://www.ebi.ac.uk/pdbsum/6j0b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j0b ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PAU_03352, PA-RVA20-21-0170 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553480 PHOAA]), PAU_03353, PA-RVA20-21-0171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553480 PHOAA])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j0b OCA], [http://pdbe.org/6j0b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j0b RCSB], [http://www.ebi.ac.uk/pdbsum/6j0b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j0b ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 20: / Line 21: @@
 </StructureSection>
 [[Category: Large Structures]]
+[[Category: Phoaa]]
 [[Category: Cai, B]]
 [[Category: Cheng, J]]

6j0b

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Revision as of 06:58, 17 April 2019

Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath-tube complex in extended state

Structural highlights

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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