2q9o

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[[Image:2q9o.jpg|left|200px]]
[[Image:2q9o.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2q9o |SIZE=350|CAPTION= <scene name='initialview01'>2q9o</scene>, resolution 1.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2q9o", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OHI:3-(2-OXO-2H-IMIDAZOL-4-YL)-L-ALANINE'>OHI</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= LAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=204285 Melanocarpus albomyces])
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00394 Cu-oxidase], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam07732 Cu-oxidase_3], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam07731 Cu-oxidase_2]</span>
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{{STRUCTURE_2q9o| PDB=2q9o | SCENE= }}
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|RELATEDENTRY=[[1gw0|1gw0]], [[2ih8|2ih8]], [[2ih9|2ih9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q9o OCA], [http://www.ebi.ac.uk/pdbsum/2q9o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q9o RCSB]</span>
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}}
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'''Near-atomic resolution structure of a Melanocarpus albomyces laccase'''
'''Near-atomic resolution structure of a Melanocarpus albomyces laccase'''
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[[Category: Rouvinen, J.]]
[[Category: Rouvinen, J.]]
[[Category: 2-oxohistidine]]
[[Category: 2-oxohistidine]]
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[[Category: laccase]]
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[[Category: Laccase]]
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[[Category: melanocarpus albomyce]]
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[[Category: Melanocarpus albomyce]]
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[[Category: multicopper oxidase]]
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[[Category: Multicopper oxidase]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 9 14:40:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:46:46 2008''
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Revision as of 11:40, 9 April 2008

Image:2q9o.jpg

Template:STRUCTURE 2q9o

Near-atomic resolution structure of a Melanocarpus albomyces laccase


Overview

We have solved a crystal structure from Melanocarpus albomyces laccase expressed in the filamentous fungus Trichoderma reesei (rMaL) at 1.3A resolution by using synchrotron radiation at 100K. At the moment, this is the highest resolution that has been attained for any multicopper oxidase. The present structure confirmed our earlier proposal regarding the dynamic behaviour of the copper cluster. Thermal ellipsoids of copper atoms indicated movements of trinuclear site coppers. The direction of the type-3 copper motion was perpendicular to the type-2 copper. In addition, the structure at 1.3A resolution allowed us to describe important solvent cavities of the enzyme and the structure is also compared with other known multicopper oxidases. T2 and T3 solvent cavities, and a putative SDS-gate, formed by Ser142, Ser510 and the C-terminal Asp556 of rMaL, are described. We also observed a 2-oxohistidine, an oxidized histidine, possibly caused by a metal-catalysed oxidation by the trinuclear site coppers. To our knowledge, this is the first time that 2-oxohistidine has been observed in a protein crystal structure.

About this Structure

2Q9O is a Single protein structure of sequence from Melanocarpus albomyces. Full crystallographic information is available from OCA.

Reference

A near atomic resolution structure of a Melanocarpus albomyces laccase., Hakulinen N, Andberg M, Kallio J, Koivula A, Kruus K, Rouvinen J, J Struct Biol. 2007 Dec 28;. PMID:18249560 Page seeded by OCA on Wed Apr 9 14:40:51 2008

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