6o1u
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of TRPV5 W583A in nanodisc== | |
| + | <StructureSection load='6o1u' size='340' side='right'caption='[[6o1u]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6o1u]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O1U FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o1u OCA], [http://pdbe.org/6o1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o1u RCSB], [http://www.ebi.ac.uk/pdbsum/6o1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o1u ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TRPV5_RABIT TRPV5_RABIT]] Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine (PubMed:12574114). Required for normal Ca(2+) reabsorption in the kidney distal convoluted tubules (By similarity). The channel is activated by low internal calcium level and the current exhibits an inward rectification (By similarity). A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay (By similarity). Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (PubMed:12574114).[UniProtKB:P69744][UniProtKB:Q9NQA5]<ref>PMID:10085067</ref> <ref>PMID:11035011</ref> <ref>PMID:12574114</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | TRPV5 (transient receptor potential vanilloid 5) is a unique calcium-selective TRP channel essential for calcium homeostasis. Unlike other TRPV channels, TRPV5 and its close homolog, TRPV6, do not exhibit thermosensitivity or ligand-dependent activation but are constitutively open at physiological membrane potentials and modulated by calmodulin (CaM) in a calcium-dependent manner. Here we report high-resolution electron cryomicroscopy structures of truncated and full-length TRPV5 in lipid nanodiscs, as well as of a TRPV5 W583A mutant and TRPV5 in complex with CaM. These structures highlight the mechanism of calcium regulation and reveal a flexible stoichiometry of CaM binding to TRPV5. | ||
| - | + | Structural insight into TRPV5 channel function and modulation.,Dang S, van Goor MK, Asarnow D, Wang Y, Julius D, Cheng Y, van der Wijst J Proc Natl Acad Sci U S A. 2019 Apr 11. pii: 1820323116. doi:, 10.1073/pnas.1820323116. PMID:30975749<ref>PMID:30975749</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6o1u" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Asarnow, D]] | [[Category: Asarnow, D]] | ||
| - | [[Category: Dang, S]] | ||
[[Category: Cheng, Y]] | [[Category: Cheng, Y]] | ||
| - | [[Category: | + | [[Category: Dang, S]] |
| - | [[Category: | + | [[Category: Goor, M K.van]] |
| - | + | ||
[[Category: Julius, D]] | [[Category: Julius, D]] | ||
| + | [[Category: Wang, Y]] | ||
| + | [[Category: Wijst, J van der]] | ||
| + | [[Category: Ion channel]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Trp channel]] | ||
Revision as of 07:04, 24 April 2019
Cryo-EM structure of TRPV5 W583A in nanodisc
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