5zf1
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Molecular structure of a novel 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm, Bombyx mori== | |
| + | <StructureSection load='5zf1' size='340' side='right'caption='[[5zf1]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5zf1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZF1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZF1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LOC101741628 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 BOMMO])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zf1 OCA], [http://pdbe.org/5zf1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zf1 RCSB], [http://www.ebi.ac.uk/pdbsum/5zf1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zf1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The enzyme 5,10-methylenetetrahydrofolate dehydrogenase (MTHFD) is essential for the production of certain amino acids (glycine, serine, and methionine) and nucleic acids (thymidylate and purine). Here, we identified a cDNA encoding this enzyme from the silkworm Bombyx mori. The recombinant B. mori MTHFD (bmMTHFD) expressed in Escherichia coli recognized 5,10-methylenetetrahydrofolate and 5,10-methenyltetrahydrofolate as substrate in the presence of NADP (+) as well as NAD (+). The bmMTHFD structure was determined at a resolution of 1.75 A by X-ray crystallography. Site-directed mutagenesis indicated that the amino acid residue Tyr49 contributed to its catalytic activity. Our findings provide insight into the mechanism underlying the activity of MTHFD from B. mori and potentially other insects and may therefore facilitate the development of inhibitors specific to MTHFD as insecticides. | ||
| - | + | Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori.,Haque MR, Higashiura A, Nakagawa A, Hirowatari A, Furuya S, Yamamoto K FEBS Open Bio. 2019 Feb 26;9(4):618-628. doi: 10.1002/2211-5463.12595., eCollection 2019 Apr. PMID:30984537<ref>PMID:30984537</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5zf1" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bommo]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Haque, R]] | [[Category: Haque, R]] | ||
| + | [[Category: Higashiura, A]] | ||
| + | [[Category: Nakagawa, A]] | ||
| + | [[Category: Yamamoto, K]] | ||
| + | [[Category: 10-methylenetetrahydrofolate dehydrogenase]] | ||
| + | [[Category: Lepidoptera]] | ||
| + | [[Category: Nadp+]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Serine]] | ||
Revision as of 09:03, 1 May 2019
Molecular structure of a novel 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm, Bombyx mori
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