1uwy

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[[Image:1uwy.jpg|left|200px]]
[[Image:1uwy.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1uwy |SIZE=350|CAPTION= <scene name='initialview01'>1uwy</scene>, resolution 3.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1uwy", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_M Carboxypeptidase M], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.12 3.4.17.12] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1uwy| PDB=1uwy | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uwy OCA], [http://www.ebi.ac.uk/pdbsum/1uwy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uwy RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF HUMAN CARBOXYPEPTIDASE M'''
'''CRYSTAL STRUCTURE OF HUMAN CARBOXYPEPTIDASE M'''
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[[Category: Reverter, D.]]
[[Category: Reverter, D.]]
[[Category: SPINE, Structural Proteomics in Europe.]]
[[Category: SPINE, Structural Proteomics in Europe.]]
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[[Category: carboxypeptidase]]
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[[Category: Carboxypeptidase]]
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[[Category: gpi-anchor]]
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[[Category: Gpi-anchor]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: lipoprotein]]
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[[Category: Lipoprotein]]
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[[Category: metallopeptidase]]
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[[Category: Metallopeptidase]]
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[[Category: metalloprotease]]
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[[Category: Metalloprotease]]
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[[Category: spine]]
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[[Category: Spine]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: structural proteomics in europe]]
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[[Category: Structural proteomics in europe]]
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[[Category: zinc]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:13:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:16:29 2008''
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Revision as of 05:13, 13 April 2008

Image:1uwy.jpg

Template:STRUCTURE 1uwy

CRYSTAL STRUCTURE OF HUMAN CARBOXYPEPTIDASE M


Overview

Carboxypeptidase M (CPM), an extracellular glycosylphosphatidyl-inositol(GPI)-anchored membrane glycoprotein belonging to the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, specifically removes C-terminal basic residues from peptides and proteins. Due to its wide distribution in human tissues, CPM is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. We have crystallized human GPI-free CPM, and have determined and refined its 3.0A crystal structure. The structure analysis reveals that CPM consists of a 295 residue N-terminal catalytic domain similar to that of duck CPD-2 (but only distantly related to CPA/B), an adjacent 86 residue beta-sandwich C-terminal domain characteristic of the CPN/E family but more conically shaped than the equivalent domain in CPD-2, and a unique, partially disordered 25 residue C-terminal extension to which the GPI membrane-anchor is post-translationally attached. Through this GPI anchor, and presumably via some positively charged side-chains of the C-terminal domain, the CPM molecule may interact with the membrane in such a way that its active centre will face alongside, i.e. well suited to interact with other membrane-bound protein substrates or small peptides. Modelling of the C-terminal part of the natural substrate Arg(6)-Met-enkephalin into the active site shows that the S1' pocket of CPM is particularly well designed to accommodate P1'-Arg residues, in agreement with the preference of CPM for cleaving C-terminal Arg.

About this Structure

1UWY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human carboxypeptidase M, a membrane-bound enzyme that regulates peptide hormone activity., Reverter D, Maskos K, Tan F, Skidgel RA, Bode W, J Mol Biol. 2004 Apr 23;338(2):257-69. PMID:15066430 Page seeded by OCA on Sun Apr 13 08:13:10 2008

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