1zj8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1zj8.gif|left|200px]]
[[Image:1zj8.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1zj8 |SIZE=350|CAPTION= <scene name='initialview01'>1zj8</scene>, resolution 2.80&Aring;
+
The line below this paragraph, containing "STRUCTURE_1zj8", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SRM:SIROHEME'>SRM</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--nitrite_reductase Ferredoxin--nitrite reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.7.1 1.7.7.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= NIRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1zj8| PDB=1zj8 | SCENE= }}
-
|RELATEDENTRY=[[1zj9|1ZJ9]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zj8 OCA], [http://www.ebi.ac.uk/pdbsum/1zj8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zj8 RCSB]</span>
+
-
}}
+
'''Structure of Mycobacterium tuberculosis NirA protein'''
'''Structure of Mycobacterium tuberculosis NirA protein'''
Line 32: Line 29:
[[Category: Schneider, G.]]
[[Category: Schneider, G.]]
[[Category: Schnell, R.]]
[[Category: Schnell, R.]]
-
[[Category: cys-tyr covalent bond]]
+
[[Category: Cys-tyr covalent bond]]
-
[[Category: fe4-s4]]
+
[[Category: Fe4-s4]]
-
[[Category: nira]]
+
[[Category: Nira]]
-
[[Category: nitrite]]
+
[[Category: Nitrite]]
-
[[Category: reductase]]
+
[[Category: Reductase]]
-
[[Category: siroheme]]
+
[[Category: Siroheme]]
-
[[Category: spine]]
+
[[Category: Spine]]
-
[[Category: structural genomic]]
+
[[Category: Structural genomic]]
-
[[Category: structural proteomics in europe]]
+
[[Category: Structural proteomics in europe]]
-
[[Category: sulfite]]
+
[[Category: Sulfite]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:16:11 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:23 2008''
+

Revision as of 05:16, 13 April 2008

Image:1zj8.gif

Template:STRUCTURE 1zj8

Structure of Mycobacterium tuberculosis NirA protein


Overview

The nirA gene of Mycobacterium tuberculosis is up-regulated in the persistent state of the bacteria, suggesting that it is a potential target for the development of antituberculosis agents particularly active against the pathogen in its dormant phase. This gene encodes a ferredoxin-dependent sulfite reductase, and the structure of the enzyme has been determined using x-ray crystallography. The enzyme is a monomer comprising 555 amino acids and contains a [Fe4-S4] cluster and a siroheme cofactor. The molecule is built up of three domains with an alpha/beta fold. The first domain consists of two ferredoxin-like subdomains, related by a pseudo-2-fold symmetry axis passing through the whole molecule. The other two domains, which provide much of the binding interactions with the cofactors, have a common fold that is unique to the sulfite/nitrite reductase family. The domains form a trilobal structure, with the cofactors and the active site located at the interface of all three domains in the center of the molecule. NirA contains an unusual covalent bond between the side chains of Tyr69 and Cys161 in the active site, in close proximity to the siroheme cofactor. Removal of this covalent bond by site-directed mutagenesis impairs catalytic activity, suggesting that it is important for the enzymatic reaction. These residues are part of a sequence fingerprint, able to distinguish between ferredoxin-dependent sulfite and nitrite reductases. Comparison of NirA with the structure of the truncated NADPH-dependent sulfite reductase from Escherichia coli suggests a binding site for the external electron donor ferredoxin close to the [Fe4-S4] cluster.

About this Structure

1ZJ8 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis is a sulfite reductase with a covalent Cys-Tyr bond in the active site., Schnell R, Sandalova T, Hellman U, Lindqvist Y, Schneider G, J Biol Chem. 2005 Jul 22;280(29):27319-28. Epub 2005 May 24. PMID:15917234 Page seeded by OCA on Sun Apr 13 08:16:11 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zj8"
Personal tools