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5n7y

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Revision as of 13:12, 10 May 2019

Solution structure of B. subtilis Sigma G inhibitor CsfB

Structural highlights

5n7y is a 2 chain structure with sequence from "vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	csfB, gin, yaaM, BSU00240 ("Vibrio subtilis" Ehrenberg 1835)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GIN_BACSU] An anti-sigma-G factor, prevents premature activation of sigma-G factor in the forespore; overexpression leads to 1000-fold reduction in spore formation, spore formation stops after engulfment (PubMed:17921305, PubMed:19497328). Overexpression also inhibits sigma-G transcription activation activity (PubMed:18208527). When both Gin and sigma-G are expressed in E.coli Gin inhibits sigma-G, strongly suggesting Gin inhibits by direct physical interaction (PubMed:19497328).^[1] ^[2] ^[3]

Publication Abstract from PubMed

Global changes in bacterial gene expression can be orchestrated by the coordinated activation/deactivation of alternative sigma (sigma) factor subunits of RNA polymerase. Sigma factors themselves are regulated in myriad ways, including via anti-sigma factors. Here, we have determined the solution structure of anti-sigma factor CsfB, responsible for inhibition of two alternative sigma factors, sigma(G) and sigma(E), during spore formation by Bacillus subtilis. CsfB assembles into a symmetrical homodimer, with each monomer bound to a single Zn(2+) ion via a treble-clef zinc finger fold. Directed mutagenesis indicates that dimer formation is critical for CsfB-mediated inhibition of both sigma(G) and sigma(E), and we have characterized these interactions in vitro. This work represents an advance in our understanding of how CsfB mediates inhibition of two alternative sigma factors to drive developmental gene expression in a bacterium.

Structural and Functional Insights into Bacillus subtilis Sigma Factor Inhibitor, CsfB.,Martinez-Lumbreras S, Alfano C, Evans NJ, Collins KM, Flanagan KA, Atkinson RA, Krysztofinska EM, Vydyanath A, Jackter J, Fixon-Owoo S, Camp AH, Isaacson RL Structure. 2018 Mar 3. pii: S0969-2126(18)30047-9. doi:, 10.1016/j.str.2018.02.007. PMID:29526435^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chary VK, Xenopoulos P, Piggot PJ. Expression of the sigmaF-directed csfB locus prevents premature appearance of sigmaG activity during sporulation of Bacillus subtilis. J Bacteriol. 2007 Dec;189(23):8754-7. doi: 10.1128/JB.01265-07. Epub 2007 Oct 5. PMID:17921305 doi:http://dx.doi.org/10.1128/JB.01265-07
↑ Karmazyn-Campelli C, Rhayat L, Carballido-Lopez R, Duperrier S, Frandsen N, Stragier P. How the early sporulation sigma factor sigmaF delays the switch to late development in Bacillus subtilis. Mol Microbiol. 2008 Mar;67(5):1169-80. doi: 10.1111/j.1365-2958.2008.06121.x., Epub 2008 Jan 15. PMID:18208527 doi:http://dx.doi.org/10.1111/j.1365-2958.2008.06121.x
↑ Rhayat L, Duperrier S, Carballido-Lopez R, Pellegrini O, Stragier P. Genetic dissection of an inhibitor of the sporulation sigma factor sigma(G). J Mol Biol. 2009 Jul 31;390(5):835-44. doi: 10.1016/j.jmb.2009.05.073. Epub 2009 , Jun 1. PMID:19497328 doi:http://dx.doi.org/10.1016/j.jmb.2009.05.073
↑ Martinez-Lumbreras S, Alfano C, Evans NJ, Collins KM, Flanagan KA, Atkinson RA, Krysztofinska EM, Vydyanath A, Jackter J, Fixon-Owoo S, Camp AH, Isaacson RL. Structural and Functional Insights into Bacillus subtilis Sigma Factor Inhibitor, CsfB. Structure. 2018 Mar 3. pii: S0969-2126(18)30047-9. doi:, 10.1016/j.str.2018.02.007. PMID:29526435 doi:http://dx.doi.org/10.1016/j.str.2018.02.007

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5n7y"

@@ Line 1: / Line 1: @@
 ==Solution structure of B. subtilis Sigma G inhibitor CsfB==
-<StructureSection load='5n7y' size='340' side='right' caption='[[5n7y]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='5n7y' size='340' side='right'caption='[[5n7y]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5n7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N7Y FirstGlance]. <br>
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Vibrio subtilis ehrenberg 1835]]
+[[Category: Large Structures]]
 [[Category: Alfano, C]]
 [[Category: Atkinson, A]]

5n7y

From Proteopedia

Revision as of 13:12, 10 May 2019

Solution structure of B. subtilis Sigma G inhibitor CsfB

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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