1ae9

From Proteopedia

(Difference between revisions)

Revision as of 13:57, 10 May 2019

STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE

Structural highlights

1ae9 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VINT_LAMBD] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Lambda integrase is archetypic of site-specific recombinases that catalyze intermolecular DNA rearrangements without energetic input. DNA cleavage, strand exchange, and religation steps are linked by a covalent phosphotyrosine intermediate in which Tyr342 is attached to the 3'-phosphate of the DNA cut site. The 1.9 angstrom crystal structure of the integrase catalytic domain reveals a protein fold that is conserved in organisms ranging from archaebacteria to yeast and that suggests a model for interaction with target DNA. The attacking Tyr342 nucleophile is located on a flexible loop about 20 angstroms from a basic groove that contains all the other catalytically essential residues. This bipartite active site can account for several apparently paradoxical features of integrase family recombinases, including the capacity for both cis and trans cleavage of DNA.

Flexibility in DNA recombination: structure of the lambda integrase catalytic core.,Kwon HJ, Tirumalai R, Landy A, Ellenberger T Science. 1997 Apr 4;276(5309):126-31. PMID:9082984^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kwon HJ, Tirumalai R, Landy A, Ellenberger T. Flexibility in DNA recombination: structure of the lambda integrase catalytic core. Science. 1997 Apr 4;276(5309):126-31. PMID:9082984

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ae9"

@@ Line 1: / Line 1: @@
 ==STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE==
-<StructureSection load='1ae9' size='340' side='right' caption='[[1ae9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='1ae9' size='340' side='right'caption='[[1ae9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1ae9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE9 FirstGlance]. <br>
@@ Line 12: / Line 12: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae9_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 29: @@
 ==See Also==
-*[[Phage integrase|Phage integrase]]
+*[[Retroviral Integrase|Retroviral Integrase]]
-*[[Resolvase|Resolvase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Ellenberger, T]]
 [[Category: Kwon, H J]]

1ae9

From Proteopedia

Revision as of 13:57, 10 May 2019

STRUCTURE OF THE LAMBDA INTEGRASE CATALYTIC CORE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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