2bf8

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[[Image:2bf8.gif|left|200px]]
[[Image:2bf8.gif|left|200px]]
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{{Structure
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|PDB= 2bf8 |SIZE=350|CAPTION= <scene name='initialview01'>2bf8</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2bf8", creates the "Structure Box" on the page.
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
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{{STRUCTURE_2bf8| PDB=2bf8 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf8 OCA], [http://www.ebi.ac.uk/pdbsum/2bf8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bf8 RCSB]</span>
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'''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K'''
'''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K'''
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[[Category: SPINE, Structural Proteomics in Europe.]]
[[Category: SPINE, Structural Proteomics in Europe.]]
[[Category: Sixma, T K.]]
[[Category: Sixma, T K.]]
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[[Category: e2 ubiquitin conjugating enzyme]]
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[[Category: E2 ubiquitin conjugating enzyme]]
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[[Category: e2-25k]]
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[[Category: E2-25k]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: spine]]
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[[Category: Spine]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: structural proteomics in europe]]
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[[Category: Structural proteomics in europe]]
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[[Category: sumo]]
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[[Category: Sumo]]
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[[Category: sumo-target structure]]
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[[Category: Sumo-target structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:17:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:05:33 2008''
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Revision as of 05:17, 13 April 2008

Image:2bf8.gif

Template:STRUCTURE 2bf8

CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K


Overview

Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.

About this Structure

2BF8 is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Full crystallographic information is available from OCA.

Reference

SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079 Page seeded by OCA on Sun Apr 13 08:17:54 2008

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