6dzc
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of Rbfox2 RRM mimetic peptide CPfox6== | |
| + | <StructureSection load='6dzc' size='340' side='right'caption='[[6dzc]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6dzc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DZC FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dzc OCA], [http://pdbe.org/6dzc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dzc RCSB], [http://www.ebi.ac.uk/pdbsum/6dzc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dzc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RNA recognition motif (RRM), which is the most abundant RNA-binding motif in eukaryotes, is a well-structured domain of about 90 amino acids, yet the beta2beta3 hairpin, corresponding to strands 2 and 3 of the beta-sheet, and the intervening loop make essential interactions with RNA in many RRM complexes. A series of small cyclic peptide mimics of the beta2beta3 hairpin of Rbfox2 protein that recognize the terminal loop of precursor miR-20b have been designed to investigate whether the full RNA-binding protein can be mimicked with a minimal structurally preorganized peptide. Within a small library of seven cyclic peptides, a peptide with low-micromolar affinity for the miR-20b precursor was found. NMR spectroscopy titration data suggest that this peptide specifically targets the apical loop of pre-miR-20b. This work shows that it is possible to mimic RNA-binding proteins with designed stable peptides, which provide a starting point for designing or evolving small peptide mimetics of RRM proteins. | ||
| - | + | A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b.,Sun YT, Shortridge MD, Varani G Chembiochem. 2019 Apr 1;20(7):931-939. doi: 10.1002/cbic.201800645. Epub 2019 Feb, 15. PMID:30537200<ref>PMID:30537200</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Sun, Y | + | <div class="pdbe-citations 6dzc" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sun, Y T]] | ||
[[Category: Varani, G]] | [[Category: Varani, G]] | ||
| + | [[Category: Peptide structure]] | ||
| + | [[Category: Peptidomimetic]] | ||
| + | [[Category: Rna binding protein]] | ||
| + | [[Category: Rna recognition]] | ||
Revision as of 07:23, 21 May 2019
Solution structure of Rbfox2 RRM mimetic peptide CPfox6
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