6gfe
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==High-resolution Structure of a therapeutic full-length anti-NPRA Antibody with exceptional Conformational Diversity== | |
| + | <StructureSection load='6gfe' size='340' side='right'caption='[[6gfe]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gfe]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GFE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GFE FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gfe OCA], [http://pdbe.org/6gfe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gfe RCSB], [http://www.ebi.ac.uk/pdbsum/6gfe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gfe ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We report the x-ray crystal structure of intact, full-length human immunoglobulin (IgG4) at 1.8 A resolution. The data for IgG4 (S228P), an antibody targeting the natriuretic peptide receptor A, show a previously unrecognized type of Fab-Fc orientation with a distorted lambda-shape in which one Fab-arm is oriented toward the Fc portion. Detailed structural analysis by x-ray crystallography and molecular simulations suggest that this is one of several conformations coexisting in a dynamic equilibrium state. These results were confirmed by small angle x-ray scattering in solution. Furthermore, electron microscopy supported these findings by preserving molecule classes of different conformations. This study fosters our understanding of IgG4 in particular and our appreciation of antibody flexibility in general. Moreover, we give insights into potential biological implications, specifically for the interaction of human anti-natriuretic peptide receptor A IgG4 with the neonatal Fc receptor, Fcgamma receptors, and complement-activating C1q by considering conformational flexibility. | ||
| - | + | Structure of a Therapeutic Full-Length Anti-NPRA IgG4 Antibody: Dissecting Conformational Diversity.,Blech M, Horer S, Kuhn AB, Kube S, Goddeke H, Kiefer H, Zang Y, Alber Y, Kast SM, Westermann M, Tully MD, Schafer LV, Garidel P Biophys J. 2019 May 7;116(9):1637-1649. doi: 10.1016/j.bpj.2019.03.036. Epub 2019, Apr 5. PMID:31023536<ref>PMID:31023536</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6gfe" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hoerer, S]] | ||
| + | [[Category: Antibody]] | ||
| + | [[Category: Immune system]] | ||
| + | [[Category: Immunoglobulin]] | ||
| + | [[Category: Natriuretic peptide receptor some]] | ||
Revision as of 07:24, 21 May 2019
High-resolution Structure of a therapeutic full-length anti-NPRA Antibody with exceptional Conformational Diversity
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