2iz1

From Proteopedia

Revision as of 16:15, 5 November 2007

6PDH COMPLEXED WITH PEX INHIBITOR SYNCHROTRON DATA

Overview

Crystal structures of recombinant Lactococcus lactis 6-phosphogluconate, dehydrogenase (LlPDH) in complex with substrate, cofactor, product and, inhibitors have been determined. LlPDH shares significant sequence, identity with the enzymes from sheep liver and the protozoan parasite, Trypanosoma brucei for which structures have been reported. Comparisons, indicate that the key residues in the active site are highly conserved, as, are the interactions with the cofactor and the product ribulose, 5-phosphate. However, there are differences in the conformation of the, substrate 6-phosphogluconate which may reflect distinct states relevant to, catalysis. Analysis of the complex formed with the potent inhibitor, 4-phospho-d-erythronohydroxamic acid, suggests that this molecule does, indeed mimic the high-energy intermediate state that it was designed to., The analysis also identified, as a contaminant by-product of the inhibitor, synthesis, 4-phospho-d-erythronamide, which binds in similar fashion., LlPDH can now serve as a model system for structure-based inhibitor design, targeting the enzyme from Trypanosoma species.

About this Structure

2IZ1 is a Single protein structure of sequence from Lactococcus lactis with CL, ATR, RES, P33 and PEG as ligands. Active as Phosphogluconate dehydrogenase (decarboxylating), with EC number 1.1.1.44 Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates., Sundaramoorthy R, Iulek J, Barrett MP, Bidet O, Ruda GF, Gilbert IH, Hunter WN, FEBS J. 2007 Jan;274(1):275-86. PMID:17222187

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