5zn8
From Proteopedia
(Difference between revisions)
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==Crystal structure of nicotinamidase PncA from Bacillus subtilis== | ==Crystal structure of nicotinamidase PncA from Bacillus subtilis== | ||
| - | <StructureSection load='5zn8' size='340' side='right' caption='[[5zn8]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='5zn8' size='340' side='right'caption='[[5zn8]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5zn8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZN8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZN8 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BMJ37_14840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zn8 OCA], [http://pdbe.org/5zn8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zn8 RCSB], [http://www.ebi.ac.uk/pdbsum/5zn8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zn8 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zn8 OCA], [http://pdbe.org/5zn8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zn8 RCSB], [http://www.ebi.ac.uk/pdbsum/5zn8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zn8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The nicotinamidase/pyrazinamidase PncA is a member of a large family of hydrolase enzymes that catalyze the deamination of nicotinamide to nicotinic acid. PncA also functions as a pyrazinamidase in a wide variety of eubacteria and is an essential coenzyme in many cellular redox reactions in living systems. We report the crystal structure of substrate-free PncA from Bacillus subtilis (BsPncA) at 2.0A resolution to improve our understanding of the PncA family. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. Our results provide useful information that will further enhance our understanding of the molecular functions of PncA family members. | ||
| + | |||
| + | Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis.,Shang F, Chen J, Wang L, Jin L, Zou L, Bu T, Dong Y, Ha NC, Nam KH, Quan C, Xu Y Biochem Biophys Res Commun. 2018 Aug 11. pii: S0006-291X(18)31749-2. doi:, 10.1016/j.bbrc.2018.08.067. PMID:30107912<ref>PMID:30107912</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5zn8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Vibrio subtilis ehrenberg 1835]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Chen, J]] | [[Category: Chen, J]] | ||
[[Category: Shang, F]] | [[Category: Shang, F]] | ||
Revision as of 08:07, 21 May 2019
Crystal structure of nicotinamidase PncA from Bacillus subtilis
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