Journal:Acta Cryst D:S2059798319006995
From Proteopedia
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<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| + | HER2, a member of the epidermal growth factor receptor (EGFR) family, has been associated with human breast, ovarian, and gastric cancers. We previously developed a chimeric antibody chA21 that specifically inhibits the growth of HER2-overexpressing cancer cells both in vitro and in vivo. To reduce potential human anti-mouse immune response, humanized antibody HuA21 was developed on the basis of chA21. | ||
| + | The crystal structure of HuA21-scFv in complex with the extracellular domain (ECD) of HER2 was determined, which demonstrates that HuA21 binds almost the same epitope as chA21 and also provides insight into how substitutions in HuA21 improve the binding affinity compared with chA21. | ||
| + | The binding epitopes of antibodies could contribute to understand their anti-tumor activities. Our HuA21-HER2 structure clearly shows that the epitope of HuA21 is located at the C-terminal part of subdomain I of HER2. This is a new epitope distinct from those described for Trastuzumab and Pertuzumab, which are located at subdomains IV and II, respectively. | ||
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 12:41, 22 May 2019
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This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
