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6irj
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of lysozyme by fixed-target serial femtosecond crystallography== | |
| + | <StructureSection load='6irj' size='340' side='right'caption='[[6irj]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6irj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IRJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IRJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6irj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6irj OCA], [http://pdbe.org/6irj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6irj RCSB], [http://www.ebi.ac.uk/pdbsum/6irj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6irj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fixed-target serial femtosecond crystallography (FT-SFX) was an important advance in crystallography by dramatically reducing sample consumption, while maintaining the benefits of SFX for obtaining crystal structures at room temperature without radiation damage. Despite a number of advantages, preparation of a sample holder for the sample delivery in FT-SFX with the use of many crystals in a single mount at ambient temperature is challenging as it can be complicated and costly, and thus, development of an efficient sample holder is essential. In this study, we introduced a nylon mesh-based sample holder enclosed by a polyimide film. This sample holder can be rapidly manufactured using a commercially available nylon mesh with pores of a desired size at a low cost without challenging technology. Furthermore, this simple device is highly efficient in data acquisition. We performed FT-SFX using a nylon mesh-based sample holder and collected over 130,000 images on a single sample holder using a 30 Hz X-ray pulse for 1.2 h. We determined the crystal structures of lysozyme and glucose isomerase using the nylon mesh at 1.65 and 1.75 A, respectively. The nylon mesh exposed to X-rays produced very low levels of background scattering at 3.75 and 4.30 A, which are negligible for data analysis. Our method provides a simple and rapid but highly efficient way to deliver samples for FT-SFX. | ||
| - | + | Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography.,Lee D, Baek S, Park J, Lee K, Kim J, Lee SJ, Chung WK, Lee JL, Cho Y, Nam KH Sci Rep. 2019 May 6;9(1):6971. doi: 10.1038/s41598-019-43485-z. PMID:31061502<ref>PMID:31061502</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Nam, K | + | <div class="pdbe-citations 6irj" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lysozyme]] | ||
| + | [[Category: Nam, K H]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Serial femtosecond crystallography]] | ||
| + | [[Category: Sfx]] | ||
Revision as of 06:49, 23 May 2019
Crystal structure of lysozyme by fixed-target serial femtosecond crystallography
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