|
|
| Line 1: |
Line 1: |
| - | | + | #REDIRECT [[6orb]] This PDB entry is obsolete and replaced by 6orb |
| - | ==Fitted model of S. pombe Mdn1 into the cryo-EM map obtained in the presence of ATP and Rbin-1==
| + | |
| - | <StructureSection load='6ees' size='340' side='right' caption='[[6ees]], [[Resolution|resolution]] 7.70Å' scene=''>
| + | |
| - | == Structural highlights ==
| + | |
| - | <table><tr><td colspan='2'>[[6ees]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EES FirstGlance]. <br>
| + | |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mdn1, SPCC737.08 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ees FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ees OCA], [http://pdbe.org/6ees PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ees RCSB], [http://www.ebi.ac.uk/pdbsum/6ees PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ees ProSAT]</span></td></tr>
| + | |
| - | </table>
| + | |
| - | == Function ==
| + | |
| - | [[http://www.uniprot.org/uniprot/MDN1_SCHPO MDN1_SCHPO]] Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus.[UniProtKB:Q12019]
| + | |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Mdn1 is an essential AAA (ATPase associated with various activities) protein that removes assembly factors from distinct precursors of the ribosomal 60S subunit. However, Mdn1's large size ( approximately 5,000 amino acid [aa]) and its limited homology to other well-studied proteins have restricted our understanding of its remodeling function. Here, we present structures for S. pombe Mdn1 in the presence of AMPPNP at up to approximately 4 A or ATP plus Rbin-1, a chemical inhibitor, at approximately 8 A resolution. These data reveal that Mdn1's MIDAS domain is tethered to its ring-shaped AAA domain through an approximately 20 nm long structured linker and a flexible approximately 500 aa Asp/Glu-rich motif. We find that the MIDAS domain, which also binds other ribosome-assembly factors, docks onto the AAA ring in a nucleotide state-specific manner. Together, our findings reveal how conformational changes in the AAA ring can be directly transmitted to the MIDAS domain and thereby drive the targeted release of assembly factors from ribosomal 60S-subunit precursors.
| + | |
| - | | + | |
| - | Structural Insights into Mdn1, an Essential AAA Protein Required for Ribosome Biogenesis.,Chen Z, Suzuki H, Kobayashi Y, Wang AC, DiMaio F, Kawashima SA, Walz T, Kapoor TM Cell. 2018 Oct 18;175(3):822-834.e18. doi: 10.1016/j.cell.2018.09.015. Epub 2018 , Oct 11. PMID:30318141<ref>PMID:30318141</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6ees" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| - | __TOC__
| + | |
| - | </StructureSection>
| + | |
| - | [[Category: Fission yeast]]
| + | |
| - | [[Category: Chen, Z]]
| + | |
| - | [[Category: DiMaio, F]]
| + | |
| - | [[Category: Kapoor, T M]]
| + | |
| - | [[Category: Suzuki, H]]
| + | |
| - | [[Category: Walz, T]]
| + | |
| - | [[Category: Wang, A C]]
| + | |
| - | [[Category: Aaa protein]]
| + | |
| - | [[Category: Mechanochemical enzyme]]
| + | |
| - | [[Category: Motor protein]]
| + | |
| - | [[Category: Ribosome assembly factor]]
| + | |
| - | [[Category: Ribosome biogenesis]]
| + | |
