6r6b
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the core Shigella flexneri type III secretion system export gate complex SctRST (Spa24/Spa9/Spa29).== | |
| + | <StructureSection load='6r6b' size='340' side='right'caption='[[6r6b]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6r6b]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R6B FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6r69|6r69]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r6b OCA], [http://pdbe.org/6r6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r6b RCSB], [http://www.ebi.ac.uk/pdbsum/6r6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r6b ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SPAP_SHIFL SPAP_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three Ipa proteins. [[http://www.uniprot.org/uniprot/SPAQ_SHIFL SPAQ_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins. [[http://www.uniprot.org/uniprot/SPAR_SHIFL SPAR_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. Here, we present the cryoelectron microscopy structure of the Salmonella Typhimurium FliP-FliQ-FliR complex at 4.2 A. None of the subunits adopt canonical integral membrane protein topologies, and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems, combined with cross-linking, localize the export gate as a core component of the periplasmic portion of the machinery. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream. | ||
| - | + | Structure of the core of the type III secretion system export apparatus.,Kuhlen L, Abrusci P, Johnson S, Gault J, Deme J, Caesar J, Dietsche T, Mebrhatu MT, Ganief T, Macek B, Wagner S, Robinson CV, Lea SM Nat Struct Mol Biol. 2018 Jul;25(7):583-590. doi: 10.1038/s41594-018-0086-9. Epub, 2018 Jul 2. PMID:29967543<ref>PMID:29967543</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6r6b" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Abrusci, P]] | ||
| + | [[Category: Deme, J C]] | ||
| + | [[Category: Johnson, S]] | ||
| + | [[Category: Kuhlen, L]] | ||
| + | [[Category: Lea, S M]] | ||
| + | [[Category: Cryo-em]] | ||
| + | [[Category: Flagella]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Protein transport]] | ||
| + | [[Category: T3ss]] | ||
| + | [[Category: Type iii secretion]] | ||
Revision as of 06:22, 29 May 2019
Structure of the core Shigella flexneri type III secretion system export gate complex SctRST (Spa24/Spa9/Spa29).
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