6jyv
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='6jyv' size='340' side='right'caption='[[6jyv]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='6jyv' size='340' side='right'caption='[[6jyv]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6jyv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JYV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jyv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JYV FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA4191 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jyv OCA], [http://pdbe.org/6jyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jyv RCSB], [http://www.ebi.ac.uk/pdbsum/6jyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jyv ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jyv OCA], [http://pdbe.org/6jyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jyv RCSB], [http://www.ebi.ac.uk/pdbsum/6jyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jyv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Isopenicillin N synthase (IPNS) is a nonheme-Fe(2+)-dependent enzyme that mediates a key step in penicillin biosynthesis. It catalyses the conversion of the tripeptide delta-(l-alpha-aminoadipoyl)-l-cysteine-d-valine (ACV) to isopenicillin N, which is a key precursor to beta-lactam antibiotics. The pa4191 gene in Pseudomonas aeruginosa PAO1 has provisionally been annotated as a member of the IPNS family. In this work, we report the crystal structure of PA4191 from P. aeruginosa (PaIPNS hereafter). The 1.65A resolution PaIPNS structure forms a jelly roll fold and is confirmed to be a member of the IPNS family based on structural homology. A metal centre within the jelly roll consists of the strictly conserved His201, Asp203 and His257 residues. MicroScale Thermophoresis binding analysis confirms that PaIPNS is a metal-binding protein with a strong preference for iron, but that it does not bind the tripeptide ACV. Structural comparison of PaIPNS with a previously reported IPNS-ACV complex structure reveals a restricted binding pocket that is unable to accommodate ACV. | ||
| + | |||
| + | Structural characterization of an isopenicillin N synthase family oxygenase from Pseudomonas aeruginosa PAO1.,Zhang H, Che S, Wang R, Liu R, Zhang Q, Bartlam M Biochem Biophys Res Commun. 2019 May 13. pii: S0006-291X(19)30932-5. doi:, 10.1016/j.bbrc.2019.05.062. PMID:31097228<ref>PMID:31097228</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6jyv" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseae]] | ||
[[Category: Bartlam, M]] | [[Category: Bartlam, M]] | ||
[[Category: Che, S]] | [[Category: Che, S]] | ||
Revision as of 06:37, 29 May 2019
Structure of an isopenicillin N synthase from Pseudomonas aeruginosa PAO1
| |||||||||||
