6mwq
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Single particle cryoEM structure of a DARPin-aldolase platform in complex with GFP== | |
| + | <StructureSection load='6mwq' size='340' side='right'caption='[[6mwq]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6mwq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MWQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences]), GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mwq OCA], [http://pdbe.org/6mwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mwq RCSB], [http://www.ebi.ac.uk/pdbsum/6mwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mwq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Solving protein structures by single-particle cryoelectron microscopy (cryo-EM) has become a crucial tool in structural biology. While exciting progress is being made toward the visualization of small macromolecules, the median protein size in both eukaryotes and bacteria is still beyond the reach of cryo-EM. To overcome this problem, we implemented a platform strategy in which a small protein target was rigidly attached to a large, symmetric base via a selectable adapter. Of our seven designs, the best construct used a designed ankyrin repeat protein (DARPin) rigidly fused to tetrameric rabbit muscle aldolase through a helical linker. The DARPin retained its ability to bind its target: GFP. We solved the structure of this complex to 3.0 A resolution overall, with 5-8 A resolution in the GFP region. As flexibility in the DARPin position limited the overall resolution of the target, we describe strategies to rigidify this element. | ||
| - | + | Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM.,Yao Q, Weaver SJ, Mock JY, Jensen GJ Structure. 2019 Apr 25. pii: S0969-2126(19)30125-X. doi:, 10.1016/j.str.2019.04.003. PMID:31080120<ref>PMID:31080120</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6mwq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aeqvi]] | ||
| + | [[Category: Fructose-bisphosphate aldolase]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct sequences]] | ||
| + | [[Category: Weaver, S J]] | ||
[[Category: Yao, Q]] | [[Category: Yao, Q]] | ||
| - | [[Category: | + | [[Category: Lyase-fluorescent protein complex]] |
| + | [[Category: Platform]] | ||
| + | [[Category: Single particle cryoem]] | ||
| + | [[Category: Small protein display]] | ||
| + | [[Category: Synthetic construct]] | ||
Revision as of 06:38, 29 May 2019
Single particle cryoEM structure of a DARPin-aldolase platform in complex with GFP
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