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Publication Abstract from PubMed

In eukaryotic and prokaryotic cells, F-ATP synthases provide energy through the synthesis of ATP. The chloroplast F-ATP synthase (CF1FO-ATP synthase) of plants is integrated into the thylakoid membrane via its FO-domain subunits a, b, b' and c Subunit c with a stoichiometry of 14 and subunit a form the gate for H(+)-pumping, enabling the coupling of electrochemical energy with ATP synthesis in the F1 sector.Here we report the crystallization and structure determination of the c14-ring of subunit c of the CF1FO-ATP synthase from spinach chloroplasts. The crystals belonged to space group C2, with unit-cell parameters a=144.420, b=99.295, c=123.51 A, and beta=104.34 degrees and diffracted to 4.5 A resolution. Each c-ring contains 14 monomers in the asymmetric unit. The length of the c-ring is 60.32 A, with an outer ring diameter 52.30 A and an inner ring width of 40 A.

Crystallographic structure of the turbine C-ring from spinach chloroplast F-ATP synthase.,Balakrishna AM, Seelert H, Marx SH, Dencher NA, Gruber G Biosci Rep. 2014 Apr 1;34(2). pii: BSR20130114. doi: 10.1042/BSR20130114. Print, 2014 Apr 1. PMID:27919036^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.