This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5zol
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a three sites mutantion of FSAA complexed with HA and product== | |
| - | + | <StructureSection load='5zol' size='340' side='right'caption='[[5zol]], [[Resolution|resolution]] 2.17Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[5zol]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZOL FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4Y8:1-HYDROXYPROPAN-2-ONE'>4Y8</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LW1:thiophene-2-carbaldehyde'>LW1</scene>, <scene name='pdbligand=LW2:(3S,4S)-3,4-dihydroxy-4-(thiophen-2-yl)butan-2-one'>LW2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |
| - | [ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zol OCA], [http://pdbe.org/5zol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zol RCSB], [http://www.ebi.ac.uk/pdbsum/5zol PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zol ProSAT]</span></td></tr> |
| - | [[Category: | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/FSAA_ECOLI FSAA_ECOLI]] Catalyzes the reversible formation of fructose 6-phosphate from dihydroxyacetone (DHA) and D-glyceraldehyde 3-phosphate via an aldolization reaction. Can utilize several aldehydes as acceptor compounds in vitro, and hydroxyacetone (HA) or 1-hydroxy-butan-2-one as alternative donor substrate. Is also able to catalyze the direct stereoselective self-aldol addition of glycolaldehyde to furnish D-(-)-threose, and cross-aldol reactions of glycolaldehyde to other aldehyde acceptors. Is not able to cleave fructose, fructose 1-phosphate, glucose 6-phosphate, sedoheptulose 1,7-bisphosphate, xylulose 5-phosphate, ribulose 5-phosphate, and fructose 1,6-bisphosphate; cannot use dihydroxyacetone phosphate as donor compound nor D-glyceraldehyde as acceptor. Does not display transaldolase activity.<ref>PMID:11120740</ref> [HAMAP-Rule:MF_00496]<ref>PMID:17985886</ref> <ref>PMID:19554584</ref> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Wu, L]] | [[Category: Wu, L]] | ||
| - | [[Category: Yu, H | + | [[Category: Yang, X H]] |
| - | [[Category: Zhou, J | + | [[Category: Yu, H W]] |
| + | [[Category: Zhou, J H]] | ||
| + | [[Category: Acceptor]] | ||
| + | [[Category: Donor]] | ||
| + | [[Category: Flexible]] | ||
| + | [[Category: Fructose-6-phosphate aldolase]] | ||
| + | [[Category: Lyase]] | ||
| + | [[Category: Mutant]] | ||
Revision as of 05:23, 12 June 2019
Crystal structure of a three sites mutantion of FSAA complexed with HA and product
| |||||||||||
Categories: Large Structures | Wu, L | Yang, X H | Yu, H W | Zhou, J H | Acceptor | Donor | Flexible | Fructose-6-phosphate aldolase | Lyase | Mutant
