4yk8

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(Difference between revisions)

Revision as of 06:44, 12 June 2019

Crystal structure of the Atg101-Atg13 complex from fission yeast

Structural highlights

4yk8 is a 2 chain structure with sequence from Fission yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	mug66, atg101, SPAC25H1.03 (Fission yeast), atg13, mug78, SPAC4F10.07c (Fission yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MUG66_SCHPO] Autophagy factor required for autophagosome formation (By similarity). Has a role in meiosis and sporulation.^[1] [ATG13_SCHPO] Activates the atg1 kinase in a nutritional condition dependent manner through the TOR pathway, leading to autophagy. Also involved in cytoplasm to vacuole transport (Cvt) and more specifically in Cvt vesicle formation. Seems to play a role in the switching machinery regulating the conversion between the Cvt pathway and autophagy. Autophagy functions to supply nitrogen and is activated when cells cannot access exogenous nitrogen, thus ensuring that they can adapt and subsequently propagate. Finally, atg13 is also required for glycogen storage during stationary phase and has a role in meiosis and sporulation.^[2] ^[3] ^[4]

Publication Abstract from PubMed

Atg101 is an essential component of the autophagy-initiating ULK complex in higher eukaryotes, but it is absent from the functionally equivalent Atg1 complex in budding yeast. Here, we report the crystal structure of the fission yeast Atg101-Atg13 complex. Atg101 has a Hop1, Rev7 and Mad2 (HORMA) architecture similar to that of Atg13. Mad2 HORMA has two distinct conformations (O-Mad2 and C-Mad2), and, intriguingly, Atg101 resembles O-Mad2 rather than the C-Mad2-like Atg13. Atg13 HORMA from higher eukaryotes possesses an inherently unstable fold, which is stabilized by Atg101 via interactions analogous to those between O-Mad2 and C-Mad2. Mutational studies revealed that Atg101 is responsible for recruiting downstream factors to the autophagosome-formation site in mammals via a newly identified WF finger. These data define the molecular functions of Atg101, providing a basis for elucidating the molecular mechanisms of mammalian autophagy initiation by the ULK complex.

Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation.,Suzuki H, Kaizuka T, Mizushima N, Noda NN Nat Struct Mol Biol. 2015 Jun 1. doi: 10.1038/nsmb.3036. PMID:26030876^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Martin-Castellanos C, Blanco M, Rozalen AE, Perez-Hidalgo L, Garcia AI, Conde F, Mata J, Ellermeier C, Davis L, San-Segundo P, Smith GR, Moreno S. A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Curr Biol. 2005 Nov 22;15(22):2056-62. PMID:16303567 doi:http://dx.doi.org/S0960-9822(05)01277-7
↑ Martin-Castellanos C, Blanco M, Rozalen AE, Perez-Hidalgo L, Garcia AI, Conde F, Mata J, Ellermeier C, Davis L, San-Segundo P, Smith GR, Moreno S. A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Curr Biol. 2005 Nov 22;15(22):2056-62. PMID:16303567 doi:http://dx.doi.org/S0960-9822(05)01277-7
↑ Kohda TA, Tanaka K, Konomi M, Sato M, Osumi M, Yamamoto M. Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes. Genes Cells. 2007 Feb;12(2):155-70. PMID:17295836 doi:http://dx.doi.org/10.1111/j.1365-2443.2007.01041.x
↑ Mukaiyama H, Kajiwara S, Hosomi A, Giga-Hama Y, Tanaka N, Nakamura T, Takegawa K. Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation. Microbiology. 2009 Dec;155(Pt 12):3816-26. Epub 2009 Sep 24. PMID:19778961 doi:http://dx.doi.org/mic.0.034389-0
↑ Suzuki H, Kaizuka T, Mizushima N, Noda NN. Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Nat Struct Mol Biol. 2015 Jun 1. doi: 10.1038/nsmb.3036. PMID:26030876 doi:http://dx.doi.org/10.1038/nsmb.3036

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4yk8"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Atg101-Atg13 complex from fission yeast==
-<StructureSection load='4yk8' size='340' side='right' caption='[[4yk8]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+<StructureSection load='4yk8' size='340' side='right'caption='[[4yk8]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4yk8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YK8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4yk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YK8 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yk8 OCA], [http://pdbe.org/4yk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yk8 RCSB], [http://www.ebi.ac.uk/pdbsum/4yk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yk8 ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mug66, atg101, SPAC25H1.03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), atg13, mug78, SPAC4F10.07c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yk8 OCA], [http://pdbe.org/4yk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yk8 RCSB], [http://www.ebi.ac.uk/pdbsum/4yk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yk8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 ==See Also==
-*[[Autophagy-related protein|Autophagy-related protein]]
+*[[Autophagy-related protein 3D structures|Autophagy-related protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Fission yeast]]
+[[Category: Large Structures]]
 [[Category: Noda, N N]]
 [[Category: Suzuki, H]]

4yk8

From Proteopedia

Revision as of 06:44, 12 June 2019

Crystal structure of the Atg101-Atg13 complex from fission yeast

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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