Dynein

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Latest structural research on Dynein has brought a different view on the behaviour of the Dynein-LC and its association with the IC. It was thought that the LCs bind cargo to the Dynein complex. Due to the fact that the IC binds at the same cleft as cargo would, there is a conflict between the two binding partners and we have to consider a new scenario for the interaction between LCs and the motor complex.
Latest structural research on Dynein has brought a different view on the behaviour of the Dynein-LC and its association with the IC. It was thought that the LCs bind cargo to the Dynein complex. Due to the fact that the IC binds at the same cleft as cargo would, there is a conflict between the two binding partners and we have to consider a new scenario for the interaction between LCs and the motor complex.
Dynein transports different cargo and to have influence on this transports could be interesting as it could be the case in stopping the transport of viral products. But as we see our previous knowledge is limited and so further research on this multisubunit protein is preferable.
Dynein transports different cargo and to have influence on this transports could be interesting as it could be the case in stopping the transport of viral products. But as we see our previous knowledge is limited and so further research on this multisubunit protein is preferable.
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 +
== 3D Structures of Dynein ==
 +
[[Dynein 3D structures]]
 +
</StructureSection>
</StructureSection>
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*Dynein light chain
*Dynein light chain
-
**[[3dvt]], [[3bri]] - DmDYNC - ''Drosophila melanogaster'' <BR />
 
-
**[[3dvh]] - DmDYNC (mutant) <BR />
 
-
**[[1rhw]] - DmDYNC - NMR<BR />
 
-
**[[1ygt]] - DmDYNC TcTex1<BR />
 
**[[2e8j]], [[2b95]], [[1z09]] - hDYNC 2A – human – NMR<BR />
**[[2e8j]], [[2b95]], [[1z09]] - hDYNC 2A – human – NMR<BR />
**[[2hz5]] - hDYNC 2A<BR />
**[[2hz5]] - hDYNC 2A<BR />
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**[[3rjs]] - DYNC – ''Toxoplasma gondii''<br />
**[[3rjs]] - DYNC – ''Toxoplasma gondii''<br />
**[[5hxl]] – rbfDYNC – rice blast fungus<br />
**[[5hxl]] – rbfDYNC – rice blast fungus<br />
 +
**[[3dvt]], [[3bri]] - DmDYNC - ''Drosophila melanogaster'' <BR />
 +
**[[3dvh]] - DmDYNC (mutant) <BR />
 +
**[[1rhw]] - DmDYNC - NMR<BR />
 +
**[[1ygt]] - DmDYNC TcTex1<BR />
*Dynein light chain complex
*Dynein light chain complex
-
**[[4qh8]], [[4qh7]] - DmDYNC + anastral spindle 2<br />
+
**[[6gzl]], [[6gzj]] - hDYNC 1 + peptide<br />
-
**[[3dvp]], [[3brl]], [[3e2b]], [[5e0l]], [[5e0m]] - DmDYNC + peptide<BR />
+
**[[2xqq]] - hDYNC 2 + peptide<br />
**[[2xqq]] - hDYNC 2 + peptide<br />
**[[3p8m]] - hDYNC 2 + GCN4<br />
**[[3p8m]] - hDYNC 2 + GCN4<br />
-
**[[3zke]], [[3zkf]] - hDYNC + NEK9 peptide<br />
+
**[[3zke]], [[3zkf]] - hDYNC 1 + NEK9 peptide<br />
-
**[[4d07]] - hDYNC + myosin peptide<br />
+
**[[4d07]] - hDYNC 2 + myosin peptide<br />
-
**[[3tq7]] – hDYNC + EB1C/EB3C<br />
+
**[[3tq7]] – hDYNC 1 + EB1C/EB3C<br />
**[[1f95]], [[1f96]] - rDYNC + peptide – NMR<BR />
**[[1f95]], [[1f96]] - rDYNC + peptide – NMR<BR />
**[[4ds1]] - yDYNC + nucleoporin Nup129 peptide <br />
**[[4ds1]] - yDYNC + nucleoporin Nup129 peptide <br />
**[[4ht6]] – yDYNC + PAC11 peptide<br />
**[[4ht6]] – yDYNC + PAC11 peptide<br />
 +
**[[4qh8]], [[4qh7]] - DmDYNC + anastral spindle 2<br />
 +
**[[3dvp]], [[3brl]], [[3e2b]], [[5e0l]], [[5e0m]] - DmDYNC + peptide<BR />
*Dynein intermediate chain
*Dynein intermediate chain
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*Dynein light+intermediate chains
*Dynein light+intermediate chains
 +
**[[5jpw]] – hDYNC - NMR<br />
 +
**[[6f1z]] – hDYNC – Cryo EM<br />
 +
**[[6b9h]] - hDYNC + protein hook homolog<br />
**[[4w7g]] – DYNC – ''Chaetomium thermophilum''<br />
**[[4w7g]] – DYNC – ''Chaetomium thermophilum''<br />
-
**[[5jpw]] – hDYNC <br />
 
-
**[[6b9h]] - hDYNC + protein hook homolog<br />
 
*Dynein heavy chain
*Dynein heavy chain
-
**[[5afr]] – yDYNC N terminal<br />
+
**[[5owo]] – hDYNC <br />
-
**[[3qmz]] – yDYNC + glutathione-S-transferase <BR />
+
**[[5ayh]] – mDYNC <br />
-
**[[4w8f]] – yDYNC/lysozyme (mutant) + AMPPNP<br />
+
-
**[[5vh9]], [[5vlj]] – yDYNC + LIS1 + ATP - CryoEM<br />
+
**[[3err]] - mDYNC microtubule-binding domain/Ser-tRNA synthetase<br />
**[[3err]] - mDYNC microtubule-binding domain/Ser-tRNA synthetase<br />
**[[3wuq]] - mDYNC stalk domain<br />
**[[3wuq]] - mDYNC stalk domain<br />
**[[3j1t]] - mDYNC + tubulin α-1B chain<br />
**[[3j1t]] - mDYNC + tubulin α-1B chain<br />
**[[3j1u]] - mDYNC + tubulin α-1B+β-2B chains<br />
**[[3j1u]] - mDYNC + tubulin α-1B+β-2B chains<br />
 +
**[[5afr]] – yDYNC N terminal<br />
 +
**[[3qmz]] – yDYNC + glutathione-S-transferase <BR />
 +
**[[4w8f]] – yDYNC/lysozyme (mutant) + AMPPNP<br />
 +
**[[5vh9]], [[5vlj]] – yDYNC + LIS1 + ATP - CryoEM<br />
**[[3vkg]], [[3vkh]] - DYNC – ''Dictyostelium discoideum''<br />
**[[3vkg]], [[3vkh]] - DYNC – ''Dictyostelium discoideum''<br />
**[[2rr7]] – CrDYNC microtubule-binding domain<br />
**[[2rr7]] – CrDYNC microtubule-binding domain<br />
**[[3j6p]] - DYNC + tubulin α-1B chain – slime mold<br />
**[[3j6p]] - DYNC + tubulin α-1B chain – slime mold<br />
-
**[[5ayh]] – mDYNC <br />
 
-
**[[5nug]] – hDYNC <br />
 
*Dynein motor domain
*Dynein motor domain
 +
**[[4rh7]] – hDYNC + ADP + ATP<br />
**[[4aki]], [[4akg]] – yDYNC + ATP<br />
**[[4aki]], [[4akg]] – yDYNC + ATP<br />
**[[4akh]] – yDYNC + AMPPNP<br />
**[[4akh]] – yDYNC + AMPPNP<br />
**[[4ai6]] – yDYNC + ADP<br />
**[[4ai6]] – yDYNC + ADP<br />
**[[3j68]], [[3j67]] – DYNC – sea urchin<br />
**[[3j68]], [[3j67]] – DYNC – sea urchin<br />
-
**[[4rh7]] – hDYNC + ADP + ATP<br />
 
*Dynein tail
*Dynein tail
-
**[[5afu]] – pDCTN subunits 2,3,5,6 + dynein tail + capping protein – CryoEM<br />
+
**[[6f1y]] – hDCTN heavy+light+intermediate – CryoEM<br />
**[[5nvs]] – hDCTN heavy+light+intermediate + LC8 + TcTex + ROBL – CryoEM<br />
**[[5nvs]] – hDCTN heavy+light+intermediate + LC8 + TcTex + ROBL – CryoEM<br />
**[[5nvu]] – hDCTN motor+heavy+light+intermediate + LC8 + TcTex + ROBL – CryoEM<br />
**[[5nvu]] – hDCTN motor+heavy+light+intermediate + LC8 + TcTex + ROBL – CryoEM<br />
**[[5nw4]] – hDCTN heavy+light+intermediate + APR1 + APR11 + ROBL + beta-actin + capping protein + dynactin + P150 + BICD2N – CryoEM<br />
**[[5nw4]] – hDCTN heavy+light+intermediate + APR1 + APR11 + ROBL + beta-actin + capping protein + dynactin + P150 + BICD2N – CryoEM<br />
 +
**[[5afu]] – pDCTN subunits 2,3,5,6 + dynein tail + capping protein - pig – CryoEM<br />
 +
**[[6f3a]] – pDYNC heavy+light+intermediate + dynactin + BICD2N - Cryo EM<br />
 +
**[[6f1u]], [[6f1t]] – pDYNC heavy+light+intermediate + dynactin + BICDR1 - Cryo EM<br />
 +
**[[6f38]] – pDYNC heavy+light+intermediate + dynactin + HOOK3 - Cryo EM<br />
}}
}}

Revision as of 09:32, 18 June 2019

Dynein light (grey, magenta, cyan and green) and intermediate (pink and yellow) chains, 3fm7

Drag the structure with the mouse to rotate

3D Structures of Dynein

Updated on 18-June-2019

References

Structure

  • Hall,Karplus&Barbar (2009), "Multivalency in the assembly of intrinsically disordered dynein intermediate chain", J.Biol.Chem. (3fm7) jbc.M109.048587

Informations

  • Williams et al. (2007), "Structural and thermodynamic characterization of a cytoplasmic dynein light chain-intermediate chain complex", PNAS no.24 vol.104 10028-10033
  • Lightcap et al. (2008), "Biochemical and Structural Characterization of the Pak1-LC8 Interaction", J.Biol.Chem. jbc.M800758200


Created with the participation of Alexander Grosse-Honebrink.

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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