4zb1

Publication Abstract from PubMed

Chromoproteins (CPs) have unique colors and can be used in biological applications. In this work, a novel blue CP with a maximum absorption peak (lambdamax) at 608 nm was identified from the carpet anemone Stichodactyla gigantea (sgBP). In vivo expression of sgBP in zebrafish would change the appearance of the fishes to have a blue color, indicating the potential biomarker function. To enhance the color properties, the crystal structure of sgBP at 2.25 A resolution was determined to allow structure-based protein engineering. Among the mutations conducted in the Gln-Tyr-Gly chromophore and chromophore environment, a S157C mutation shifted the lambdamax to 604 nm with an extinction coefficient (epsilon) of 58,029 M-1.cm-1 and darkened the blue color expression. The S157C mutation in the sgBP chromophore environment could affect the color expression by altering the deprotonation state of the phenolic group in the chromophore. Our results provide a structural basis for the blue color enhancement of the biomarker development.

Chromophore Deprotonation State Alters the Optical Properties of Blue Chromoprotein.,Chiang CY, Lee CC, Lo SY, Wang AH, Tsai HJ PLoS One. 2015 Jul 28;10(7):e0134108. doi: 10.1371/journal.pone.0134108., eCollection 2015. PMID:26218063^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.