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4zkj

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Revision as of 08:47, 26 June 2019

Crystal structure of CRISPR-associated protein

Structural highlights

4zkj is a 2 chain structure with sequence from Streptococcus pyogenes m1 476. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Gene:	cas1, M1GAS476_0831 (Streptococcus pyogenes M1 476)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[J7M1H5_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.[HAMAP-Rule:MF_01470]

Publication Abstract from PubMed

CRISPRs and Cas proteins constitute an RNA-guided microbial immune system against invading nucleic acids. Cas1 is a universal Cas protein found in all three types of CRISPR-Cas systems, and its role is implicated in new spacer acquisition during CRISPR-mediated adaptive immunity. Here, we report the crystal structure of Streptococcus pyogenes Cas1 (SpCas1) in a type II CRISPR-Cas system and characterize its interaction with S. pyogenes Csn2 (SpCsn2). The SpCas1 structure reveals a unique conformational state distinct from type I Cas1 structures, resulting in a more extensive dimerization interface, a more globular overall structure, and a disruption of potential metal-binding sites for catalysis. We demonstrate that SpCas1 directly interacts with SpCsn2, and identify the binding interface and key residues for Cas complex formation. These results provide structural information for a type II Cas1 protein, and lay a foundation for studying multiprotein Cas complexes functioning in type II CRISPR-Cas systems.

Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System.,Ka D, Lee H, Jung YD, Kim K, Seok C, Suh N, Bae E Structure. 2016 Jan 5;24(1):70-9. doi: 10.1016/j.str.2015.10.019. Epub 2015 Dec, 3. PMID:26671707^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ka D, Lee H, Jung YD, Kim K, Seok C, Suh N, Bae E. Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System. Structure. 2016 Jan 5;24(1):70-9. doi: 10.1016/j.str.2015.10.019. Epub 2015 Dec, 3. PMID:26671707 doi:http://dx.doi.org/10.1016/j.str.2015.10.019

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4zkj"

@@ Line 1: / Line 1: @@
 ==Crystal structure of CRISPR-associated protein==
-<StructureSection load='4zkj' size='340' side='right' caption='[[4zkj]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='4zkj' size='340' side='right'caption='[[4zkj]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zkj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZKJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zkj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_m1_476 Streptococcus pyogenes m1 476]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZKJ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zkj OCA], [http://pdbe.org/4zkj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zkj RCSB], [http://www.ebi.ac.uk/pdbsum/4zkj PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas1, M1GAS476_0831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1207470 Streptococcus pyogenes M1 476])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zkj OCA], [http://pdbe.org/4zkj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zkj RCSB], [http://www.ebi.ac.uk/pdbsum/4zkj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zkj ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 20: @@
 </div>
 <div class="pdbe-citations 4zkj" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptococcus pyogenes m1 476]]
 [[Category: Bae, E]]
 [[Category: Ka, D]]

4zkj

From Proteopedia

Revision as of 08:47, 26 June 2019

Crystal structure of CRISPR-associated protein

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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