3brf
From Proteopedia
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'''CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221''' | '''CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221''' | ||
| + | |||
| + | ==Overview== | ||
| + | The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro. | ||
==About this Structure== | ==About this Structure== | ||
3BRF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRF OCA]. | 3BRF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRF OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18381292 18381292] | ||
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Kovall, R A.]] | [[Category: Kovall, R A.]] | ||
[[Category: Wilson, J J.]] | [[Category: Wilson, J J.]] | ||
| - | [[Category: | + | [[Category: Ank repeat]] |
| - | [[Category: | + | [[Category: Developmental protein]] |
| - | [[Category: | + | [[Category: Differentiation]] |
| - | [[Category: | + | [[Category: Dna binding protein/dna complex]] |
| - | [[Category: | + | [[Category: Dna-binding]] |
| - | [[Category: | + | [[Category: Egf-like domain]] |
| - | [[Category: | + | [[Category: Glycoprotein]] |
| - | [[Category: | + | [[Category: Membrane]] |
| - | [[Category: | + | [[Category: Notch]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | [[Category: | + | [[Category: Signaling]] |
| - | [[Category: | + | [[Category: Transcription]] |
| - | [[Category: | + | [[Category: Transmembrane]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:33:19 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 06:33, 24 April 2008
CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221
Overview
The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
About this Structure
3BRF is a Protein complex structure of sequences from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:18381292 Page seeded by OCA on Thu Apr 24 09:33:19 2008
Categories: Caenorhabditis elegans | Protein complex | Kovall, R A. | Wilson, J J. | Ank repeat | Developmental protein | Differentiation | Dna binding protein/dna complex | Dna-binding | Egf-like domain | Glycoprotein | Membrane | Notch | Protein-dna complex | Signaling | Transcription | Transmembrane
