2jgu
From Proteopedia
OCA (Talk | contribs)
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_2jgu", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Next diff →
Revision as of 06:37, 24 April 2008
CRYSTAL STRUCTURE OF DNA-DIRECTED DNA POLYMERASE
Overview
We have determined a 2.6A resolution crystal structure of Pfu DNA polymerase, the most commonly used high fidelity PCR enzyme, from Pyrococcus furiosus. Although the structures of Pfu and KOD1 are highly similar, the structure of Pfu elucidates the electron density of the interface between the exonuclease and thumb domains, which has not been previously observed in the KOD1 structure. The interaction of these two domains is known to coordinate the proofreading and polymerization activity of DNA polymerases, especially via H147 that is present within the loop (residues 144-158) of the exonuclease domain. In our structure of Pfu, however, E148 rather than H147 is located at better position to interact with the thumb domain. In addition, the structural analysis of Pfu and KOD1 shows that both the Y-GG/A and beta-hairpin motifs of Pfu are found to differ with that of KOD1, and may explain differences in processivity. This information enables us to better understand the mechanisms of polymerization and proofreading of DNA polymerases.
About this Structure
2JGU is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus., Kim SW, Kim DU, Kim JK, Kang LW, Cho HS, Int J Biol Macromol. 2008 May 1;42(4):356-61. Epub 2008 Feb 12. PMID:18355915 Page seeded by OCA on Thu Apr 24 09:37:13 2008
