This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6gzk
From Proteopedia
(Difference between revisions)
m (Protected "6gzk" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Solution NMR structure of the tetramethylrhodamine (TMR) aptamer 3 in complex with 5-TAMRA== | |
| + | <StructureSection load='6gzk' size='340' side='right'caption='[[6gzk]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gzk]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GZK FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FH8:5-carboxy+methylrhodamine'>FH8</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gzk OCA], [http://pdbe.org/6gzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gzk RCSB], [http://www.ebi.ac.uk/pdbsum/6gzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gzk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RNA aptamers are used in a wide range of biotechnological or biomedical applications. In many cases the high resolution structures of these aptamers in their ligand-complexes have revealed fundamental aspects of RNA folding and RNA small molecule interactions. Fluorescent RNA-ligand complexes in particular find applications as optical sensors or as endogenous fluorescent tags for RNA tracking in vivo. Structures of RNA aptamers and aptamer ligand complexes constitute the starting point for rational function directed optimization approaches. Here, we present the NMR resonance assignment of an RNA aptamer binding to the fluorescent ligand tetramethylrhodamine (TMR) in complex with the ligand 5-carboxy-tetramethylrhodamine (5-TAMRA) as a starting point for a high-resolution structure determination using NMR spectroscopy in solution. | ||
| - | + | NMR resonance assignments for the tetramethylrhodamine binding RNA aptamer 3 in complex with the ligand 5-carboxy-tetramethylrhodamine.,Duchardt-Ferner E, Juen M, Kreutz C, Wohnert J Biomol NMR Assign. 2017 Apr;11(1):29-34. doi: 10.1007/s12104-016-9715-6. Epub, 2016 Oct 11. PMID:27730489<ref>PMID:27730489</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6gzk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Duchardt-Ferner, E]] | [[Category: Duchardt-Ferner, E]] | ||
| - | [[Category: Kreutz, C | + | [[Category: Kreutz, C R]] |
[[Category: Ohlenschlager, O]] | [[Category: Ohlenschlager, O]] | ||
[[Category: Wohnert, J]] | [[Category: Wohnert, J]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Rna aptamer]] | ||
| + | [[Category: Rna-ligand complex]] | ||
| + | [[Category: Three-way junction]] | ||
Revision as of 10:38, 17 July 2019
Solution NMR structure of the tetramethylrhodamine (TMR) aptamer 3 in complex with 5-TAMRA
| |||||||||||
