| Structural highlights
3mwy is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | |
| Related: | 2h1e, 2b2w, 1z63, 1z3i, 3dmq, 2db3 |
| Gene: | CHD1, SYGP-ORF4, YER164W (ATCC 18824) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[CHD1_YEAST] ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complexes SAGA and SLIK. It recognizes H3K4me. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. Acts in opposition to the FACT complex in regulating polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the pol I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome.[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Chromatin remodelers are ATP-driven machines that assemble, slide, and remove nucleosomes from DNA, but how the ATPase motors of remodelers are regulated is poorly understood. Here we show that the double chromodomain unit of the Chd1 remodeler blocks DNA binding and activation of the ATPase motor in the absence of nucleosome substrates. The Chd1 crystal structure reveals that an acidic helix joining the chromodomains can pack against a DNA-binding surface of the ATPase motor. Disruption of the chromodomain-ATPase interface prevents discrimination between nucleosomes and naked DNA and reduces the reliance on the histone H4 tail for nucleosome sliding. We propose that the chromodomains allow Chd1 to distinguish between nucleosomes and naked DNA by physically gating access to the ATPase motor, and we hypothesize that related ATPase motors may employ a similar strategy to discriminate among DNA-containing substrates.
The chromodomains of the Chd1 chromatin remodeler regulate DNA access to the ATPase motor.,Hauk G, McKnight JN, Nodelman IM, Bowman GD Mol Cell. 2010 Sep 10;39(5):711-23. PMID:20832723[12]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Grant PA, Eberharter A, John S, Cook RG, Turner BM, Workman JL. Expanded lysine acetylation specificity of Gcn5 in native complexes. J Biol Chem. 1999 Feb 26;274(9):5895-900. PMID:10026213
- ↑ Tran HG, Steger DJ, Iyer VR, Johnson AD. The chromo domain protein chd1p from budding yeast is an ATP-dependent chromatin-modifying factor. EMBO J. 2000 May 15;19(10):2323-31. PMID:10811623 doi:http://dx.doi.org/10.1093/emboj/19.10.2323
- ↑ Simic R, Lindstrom DL, Tran HG, Roinick KL, Costa PJ, Johnson AD, Hartzog GA, Arndt KM. Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes. EMBO J. 2003 Apr 15;22(8):1846-56. PMID:12682017 doi:http://dx.doi.org/10.1093/emboj/cdg179
- ↑ Robinson KM, Schultz MC. Replication-independent assembly of nucleosome arrays in a novel yeast chromatin reconstitution system involves antisilencing factor Asf1p and chromodomain protein Chd1p. Mol Cell Biol. 2003 Nov;23(22):7937-46. PMID:14585955
- ↑ Pray-Grant MG, Daniel JA, Schieltz D, Yates JR 3rd, Grant PA. Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation. Nature. 2005 Jan 27;433(7024):434-8. Epub 2005 Jan 12. PMID:15647753 doi:http://dx.doi.org/nature03242
- ↑ Stockdale C, Flaus A, Ferreira H, Owen-Hughes T. Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes. J Biol Chem. 2006 Jun 16;281(24):16279-88. Epub 2006 Apr 10. PMID:16606615 doi:http://dx.doi.org/10.1074/jbc.M600682200
- ↑ Xella B, Goding C, Agricola E, Di Mauro E, Caserta M. The ISWI and CHD1 chromatin remodelling activities influence ADH2 expression and chromatin organization. Mol Microbiol. 2006 Mar;59(5):1531-41. PMID:16468993 doi:http://dx.doi.org/10.1111/j.1365-2958.2005.05031.x
- ↑ Ferreira H, Flaus A, Owen-Hughes T. Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms. J Mol Biol. 2007 Nov 30;374(3):563-79. Epub 2007 Sep 26. PMID:17949749 doi:http://dx.doi.org/10.1016/j.jmb.2007.09.059
- ↑ Biswas D, Dutta-Biswas R, Stillman DJ. Chd1 and yFACT act in opposition in regulating transcription. Mol Cell Biol. 2007 Sep;27(18):6279-87. Epub 2007 Jul 9. PMID:17620414 doi:http://dx.doi.org/10.1128/MCB.00978-07
- ↑ Jones HS, Kawauchi J, Braglia P, Alen CM, Kent NA, Proudfoot NJ. RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nat Struct Mol Biol. 2007 Feb;14(2):123-30. Epub 2007 Jan 28. PMID:17259992 doi:http://dx.doi.org/10.1038/nsmb1199
- ↑ Biswas D, Takahata S, Xin H, Dutta-Biswas R, Yu Y, Formosa T, Stillman DJ. A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae. Genetics. 2008 Feb;178(2):649-59. doi: 10.1534/genetics.107.084202. Epub 2008 Feb, 1. PMID:18245327 doi:http://dx.doi.org/10.1534/genetics.107.084202
- ↑ Hauk G, McKnight JN, Nodelman IM, Bowman GD. The chromodomains of the Chd1 chromatin remodeler regulate DNA access to the ATPase motor. Mol Cell. 2010 Sep 10;39(5):711-23. PMID:20832723 doi:10.1016/j.molcel.2010.08.012
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