4jhd

From Proteopedia

(Difference between revisions)

Revision as of 12:02, 17 July 2019

Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu

Structural highlights

4jhd is a 6 chain structure with sequence from Drome and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	Act5C, CG4027 (DROME), Cobl, Kiaa0633 (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACT1_DROME] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction. [COBL_MOUSE] Plays an important role in the reorganization of the actin cytoskeleton. Binds to and sequesters actin monomers (G actin). Nucleates actin polymerization by assembling three actin monomers in cross-filament orientation and thereby promotes growth of actin filaments at the barbed end. Can also mediate actin depolymerization at barbed ends and severing of actin filaments. Promotes formation of cell ruffles. Regulates neuron morphogenesis and increases branching of axons and dendrites. Regulates dendrite branching in Purkinje cells.^[1] ^[2]

Publication Abstract from PubMed

Spontaneous nucleation of actin is very inefficient in cells. To overcome this barrier, cells have evolved a set of actin filament nucleators to promote rapid nucleation and polymerization in response to specific stimuli. However, the molecular mechanism of actin nucleation remains poorly understood. This is hindered largely by the fact that actin nucleus, once formed, rapidly polymerizes into filament, thus making it impossible to capture stable multisubunit actin nucleus. Here, we report an effective double-mutant strategy to stabilize actin nucleus by preventing further polymerization. Employing this strategy, we solved the crystal structure of AMPPNP-actin in complex with the first two tandem W domains of Cordon-bleu (Cobl), a potent actin filament nucleator. Further sequence comparison and functional studies suggest that the nucleation mechanism of Cobl is probably shared by the p53 cofactor JMY, but not Spire. Moreover, the double-mutant strategy opens the way for atomic mechanistic study of actin nucleation and polymerization.

Structural Basis of Actin Filament Nucleation by Tandem W Domains.,Chen X, Ni F, Tian X, Kondrashkina E, Wang Q, Ma J Cell Rep. 2013 May 29. pii: S2211-1247(13)00210-6. doi:, 10.1016/j.celrep.2013.04.028. PMID:23727244^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ahuja R, Pinyol R, Reichenbach N, Custer L, Klingensmith J, Kessels MM, Qualmann B. Cordon-bleu is an actin nucleation factor and controls neuronal morphology. Cell. 2007 Oct 19;131(2):337-50. PMID:17956734 doi:10.1016/j.cell.2007.08.030
↑ Haag N, Schwintzer L, Ahuja R, Koch N, Grimm J, Heuer H, Qualmann B, Kessels MM. The actin nucleator Cobl is crucial for Purkinje cell development and works in close conjunction with the F-actin binding protein Abp1. J Neurosci. 2012 Dec 5;32(49):17842-56. doi: 10.1523/JNEUROSCI.0843-12.2012. PMID:23223303 doi:10.1523/JNEUROSCI.0843-12.2012
↑ Chen X, Ni F, Tian X, Kondrashkina E, Wang Q, Ma J. Structural Basis of Actin Filament Nucleation by Tandem W Domains. Cell Rep. 2013 May 29. pii: S2211-1247(13)00210-6. doi:, 10.1016/j.celrep.2013.04.028. PMID:23727244 doi:10.1016/j.celrep.2013.04.028

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4jhd"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu==
-<StructureSection load='4jhd' size='340' side='right' caption='[[4jhd]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
+<StructureSection load='4jhd' size='340' side='right'caption='[[4jhd]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4jhd]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JHD FirstGlance]. <br>
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Actin|Actin]]
+*[[Actin 3D structures|Actin 3D structures]]
 == References ==
 <references/>
@@ Line 27: / Line 27: @@
 </StructureSection>
 [[Category: Drome]]
+[[Category: Large Structures]]
 [[Category: Lk3 transgenic mice]]
 [[Category: Chen, X]]

4jhd

From Proteopedia

Revision as of 12:02, 17 July 2019

Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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