6gp1
From Proteopedia
(Difference between revisions)
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<StructureSection load='6gp1' size='340' side='right'caption='[[6gp1]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='6gp1' size='340' side='right'caption='[[6gp1]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6gp1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GP1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6gp1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GP1 FirstGlance]. <br> |
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IEY:2-((1E)-2-(5-IMIDAZOLYL)ETHENYL)-4-(P-HYDROXYBENZYLIDENE)-5-IMIDAZOLINONE'>IEY</scene>, <scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IEY:2-((1E)-2-(5-IMIDAZOLYL)ETHENYL)-4-(P-HYDROXYBENZYLIDENE)-5-IMIDAZOLINONE'>IEY</scene>, <scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6gp0|6gp0]], [[6goz|6goz]], [[6goy|6goy]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6gp0|6gp0]], [[6goz|6goz]], [[6goy|6goy]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gp1 OCA], [http://pdbe.org/6gp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gp1 RCSB], [http://www.ebi.ac.uk/pdbsum/6gp1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gp1 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gp1 OCA], [http://pdbe.org/6gp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gp1 RCSB], [http://www.ebi.ac.uk/pdbsum/6gp1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gp1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort. | ||
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| + | Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.,De Zitter E, Thedie D, Monkemoller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D Nat Methods. 2019 Jul 8. pii: 10.1038/s41592-019-0462-3. doi:, 10.1038/s41592-019-0462-3. PMID:31285624<ref>PMID:31285624</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6gp1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Lobhe]] | ||
[[Category: Adam, V]] | [[Category: Adam, V]] | ||
[[Category: Bourgeois, D]] | [[Category: Bourgeois, D]] | ||
Revision as of 12:32, 17 July 2019
Structure of mEos4b in the red long-lived dark state
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