6o1o
From Proteopedia
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<StructureSection load='6o1o' size='340' side='right'caption='[[6o1o]], [[Resolution|resolution]] 3.80Å' scene=''> | <StructureSection load='6o1o' size='340' side='right'caption='[[6o1o]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6o1o]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O1O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O1O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6o1o]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O1O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O1O FirstGlance]. <br> |
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o1o OCA], [http://pdbe.org/6o1o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o1o RCSB], [http://www.ebi.ac.uk/pdbsum/6o1o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o1o ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o1o OCA], [http://pdbe.org/6o1o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o1o RCSB], [http://www.ebi.ac.uk/pdbsum/6o1o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o1o ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type III-A CRISPR-Cas systems are prokaryotic RNA-guided adaptive immune systems that use a protein-RNA complex, Csm, for transcription-dependent immunity against foreign DNA. Csm can cleave RNA and single-stranded DNA (ssDNA), but whether it targets one or both nucleic acids during transcription elongation is unknown. Here, we show that binding of a Thermus thermophilus (T. thermophilus) Csm (TthCsm) to a nascent transcript in a transcription elongation complex (TEC) promotes tethering but not direct contact of TthCsm with RNA polymerase (RNAP). Biochemical experiments show that both TthCsm and Staphylococcus epidermidis (S. epidermidis) Csm (SepCsm) cleave RNA transcripts, but not ssDNA, at the transcription bubble. Taken together, these results suggest that Type III systems primarily target transcripts, instead of unwound ssDNA in TECs, for immunity against double-stranded DNA (dsDNA) phages and plasmids. This reveals similarities between Csm and eukaryotic RNA interference, which also uses RNA-guided RNA targeting to silence actively transcribed genes. | ||
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| + | Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble.,Liu TY, Liu JJ, Aditham AJ, Nogales E, Doudna JA Nat Commun. 2019 Jul 5;10(1):3001. doi: 10.1038/s41467-019-10780-2. PMID:31278272<ref>PMID:31278272</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6o1o" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Flavobacterium thermophilum yoshida and oshima 1971]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Liu, J J]] | [[Category: Liu, J J]] | ||
Revision as of 12:39, 17 July 2019
Cryo-EM structure of the T. thermophilus Csm complex bound to target ssRNA
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