6r1j
From Proteopedia
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<StructureSection load='6r1j' size='340' side='right'caption='[[6r1j]], [[Resolution|resolution]] 1.92Å' scene=''> | <StructureSection load='6r1j' size='340' side='right'caption='[[6r1j]], [[Resolution|resolution]] 1.92Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6r1j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R1J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6r1j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila_j-1 Aeromonas hydrophila j-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R1J FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">V469_08880 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1419584 Aeromonas hydrophila J-1])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r1j OCA], [http://pdbe.org/6r1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r1j RCSB], [http://www.ebi.ac.uk/pdbsum/6r1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r1j ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r1j OCA], [http://pdbe.org/6r1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r1j RCSB], [http://www.ebi.ac.uk/pdbsum/6r1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r1j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The alpha helical CytolysinA family of pore forming toxins (alpha-PFT) contains single, two, and three component members. Structures of the single component Eschericia coli ClyA and the two component Yersinia enterolytica YaxAB show both undergo conformational changes from soluble to pore forms, and oligomerization to produce the active pore. Here we identify tripartite alpha-PFTs in pathogenic Gram negative bacteria, including Aeromonas hydrophila (AhlABC). We show that the AhlABC toxin requires all three components for maximal cell lysis. We present structures of pore components which describe a bi-fold hinge mechanism for soluble to pore transition in AhlB and a contrasting tetrameric assembly employed by soluble AhlC to hide their hydrophobic membrane associated residues. We propose a model of pore assembly where the AhlC tetramer dissociates, binds a single membrane leaflet, recruits AhlB promoting soluble to pore transition, prior to AhlA binding to form the active hydrophilic lined pore. | ||
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| + | (Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila).,Wilson JS, Churchill-Angus AM, Davies SP, Sedelnikova SE, Tzokov SB, Rafferty JB, Bullough PA, Bisson C, Baker PJ Nat Commun. 2019 Jul 1;10(1):2900. doi: 10.1038/s41467-019-10777-x. PMID:31263098<ref>PMID:31263098</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6r1j" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Aeromonas hydrophila j-1]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Baker, P J]] | [[Category: Baker, P J]] | ||
Revision as of 12:45, 17 July 2019
Structure of the soluble AhlC triple head mutant of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
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