6hn7
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.== | |
| - | + | <StructureSection load='6hn7' size='340' side='right'caption='[[6hn7]], [[Resolution|resolution]] 3.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[6hn7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HN7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HN7 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hn7 OCA], [http://pdbe.org/6hn7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hn7 RCSB], [http://www.ebi.ac.uk/pdbsum/6hn7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hn7 ProSAT]</span></td></tr> | |
| - | [[Category: | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TERS_LAMBD TERS_LAMBD]] Component of the terminase that processes and encapsidates viral genomes during virion assembly. The terminase is composed of two small and one large subunits. To initiate packaging, it binds a specific sequence called cos, at the junction of adjacent viral genomes in the concatemeric DNA substrate. Next, in a reaction stimulated by the presence of proheads and ATP but not requiring ATP hydrolysis, the terminase creates two nicks 12bp appart at the cos site, one on each stand. Terminase then separates the cohesive ends in a reaction requiring ATP hydrolysis. The heterotrimer remains bound to the left end of the genome to be packaged, forming a stable DNA-protein complex known as complex I. In a reaction facilitated by a viral assembly catalyst, gpFI, complex I binds a prohead, a preformed head shell precursor, to form complex II. In another packaging reaction requiring ATP hydrolysis, the DNA is translocated into the prohead until the next cos site on the concatemer reaches the packaging complex. At this time the downstream cos site is cut and the heterotrimer undocks from the DNA-filled head to remain bound to the left end of concatemer's next genome. The new heterotrimer-DNA complex I binds another prohead to continue the processive, polarized packaging of viral genomes. The terminase is dependent upon host integration host factor (ihfA/ihfB) for these activities.<ref>PMID:2989542</ref> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Alqasmi, M]] | ||
| + | [[Category: Bacarizo, J]] | ||
| + | [[Category: Ciges-Tomas, J R]] | ||
| + | [[Category: Fillol-Salom, A]] | ||
| + | [[Category: Marina, A]] | ||
| + | [[Category: Penades, J R]] | ||
| + | [[Category: Roszak, A W]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Hetero-dimer]] | ||
| + | [[Category: Phage interference]] | ||
| + | [[Category: Protein complex]] | ||
| + | [[Category: Redirecting packaging protein]] | ||
Revision as of 06:15, 31 July 2019
Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.
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