3jtd

From Proteopedia

(Difference between revisions)

Revision as of 06:46, 7 August 2019

Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation

Structural highlights

3jtd is a 3 chain structure with sequence from Argir and Argopecten irradians. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MYS_ARGIR] Muscle contraction. Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin. [MLE_ARGIR] In molluscan muscle, calcium regulation is associated with myosin rather than with actin. Muscle myosin contains two types of light chains: the catalytic light chain, essential for ATPase activity, and the regulatory light chain, a calcium-binding protein responsible for Ca(2+) dependent binding and Ca(2+) dependent Mg-ATPase activity. [MLR_ARGIR] In molluscan muscle, calcium regulation is associated with myosin rather than with actin. Muscle myosin contains two types of light chains: the catalytic light chain, essential for ATPase activity, and the regulatory light chain, a calcium-binding protein responsible for Ca(2+) dependent binding and Ca(2+) dependent Mg-ATPase activity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In regulated myosin, motor and enzymatic activities are toggled between the on-state and off-state by a switch located on its lever arm domain, here called the regulatory domain (RD). This region consists of a long alpha-helical "heavy chain" stabilized by a "regulatory" light chain (RLC) and an "essential" light chain (ELC). The on-state is activated by phosphorylation of the RLC of vertebrate smooth muscle RD or by direct binding of Ca(2+) to the ELC of molluscan RD. Crystal structures are available only for the molluscan RD. To understand in more detail the pathway between the on-state and the off-state, we have now also determined the crystal structure of a molluscan (scallop) RD in the absence of Ca(2+). Our results indicate that loss of Ca(2+) abolishes most of the interactions between the light chains and may increase the flexibility of the RD heavy chain. We propose that disruption of critical links with the C-lobe of the RLC is the key event initiating the off-state in both smooth muscle myosins and molluscan myosins.

The on-off switch in regulated myosins: different triggers but related mechanisms.,Himmel DM, Mui S, O'Neall-Hennessey E, Szent-Gyorgyi AG, Cohen C J Mol Biol. 2009 Dec 4;394(3):496-505. Epub 2009 Sep 19. PMID:19769984^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Himmel DM, Mui S, O'Neall-Hennessey E, Szent-Gyorgyi AG, Cohen C. The on-off switch in regulated myosins: different triggers but related mechanisms. J Mol Biol. 2009 Dec 4;394(3):496-505. Epub 2009 Sep 19. PMID:19769984 doi:10.1016/j.jmb.2009.09.035

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3jtd"

@@ Line 1: / Line 1: @@
 ==Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation==
-<StructureSection load='3jtd' size='340' side='right' caption='[[3jtd]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
+<StructureSection load='3jtd' size='340' side='right'caption='[[3jtd]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[3jtd]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Argir Argir] and [http://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JTD FirstGlance]. <br>
@@ Line 13: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jtd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jtd_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 28: / Line 28: @@
 </div>
 <div class="pdbe-citations 3jtd" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Myosin|Myosin]]
 == References ==
 <references/>
@@ Line 34: / Line 37: @@
 [[Category: Argir]]
 [[Category: Argopecten irradians]]
+[[Category: Large Structures]]
 [[Category: Cohen, C]]
 [[Category: Himmel, D M]]

3jtd

From Proteopedia

Revision as of 06:46, 7 August 2019

Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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