6p29
From Proteopedia
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<StructureSection load='6p29' size='340' side='right'caption='[[6p29]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='6p29' size='340' side='right'caption='[[6p29]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6p29]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P29 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6p29]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"alteromonas_luteo-violaceus"_(sic)_gauthier_1976 "alteromonas luteo-violaceus" (sic) gauthier 1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P29 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NQ7:(5S)-2-amino-5-[(1R)-1-(1H-indol-3-yl)ethyl]-1,3-oxazol-4(5H)-one'>NQ7</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NQ7:(5S)-2-amino-5-[(1R)-1-(1H-indol-3-yl)ethyl]-1,3-oxazol-4(5H)-one'>NQ7</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N473_05385 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43657 "Alteromonas luteo-violaceus" (sic) Gauthier 1976])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p29 OCA], [http://pdbe.org/6p29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p29 RCSB], [http://www.ebi.ac.uk/pdbsum/6p29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p29 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p29 OCA], [http://pdbe.org/6p29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p29 RCSB], [http://www.ebi.ac.uk/pdbsum/6p29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p29 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Microbes produce specialized metabolites to thrive in their natural habitats. However, it is rare that a given specialized metabolite is biosynthesized via pathways with distinct intermediates and enzymes. Here, we show that the core assembly mechanism of the antibiotic indolmycin in marine gram-negative Pseudoalteromonas luteoviolacea is distinct from its counterpart in terrestrial gram-positive Streptomyces species, with a molecule that is a shunt product in the Streptomyces pathway employed as a biosynthetic substrate for a novel metal-independent N-demethylindolmycin synthase in the P. luteoviolacea pathway. To provide insight into this reaction, we solved the 1.5 A resolution structure in complex with product and identified the active site residues. Guided by our biosynthetic insights, we then engineered the Streptomyces indolmycin producer for titer improvement. This study provides a paradigm for understanding how two unique routes to a microbial specialized metabolite can emerge from convergent biosynthetic transformations. | ||
| + | |||
| + | Convergent biosynthetic transformations to a bacterial specialized metabolite.,Du YL, Higgins MA, Zhao G, Ryan KS Nat Chem Biol. 2019 Aug 12. pii: 10.1038/s41589-019-0331-5. doi:, 10.1038/s41589-019-0331-5. PMID:31406372<ref>PMID:31406372</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6p29" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 16:04, 28 August 2019
N-demethylindolmycin synthase (PluN2) in complex with N-demethylindolmycin
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