Insulin-Degrading Enzyme

Function

Insulin-Degrading Enzyme (PDB entry 2g49) is a highly conserved 2*113 kDa homodimeric zinc metalloprotease. It belongs to the M16A family of metalloproteases within the ME clan. It hydrolyses insulin and several amyloidogenic peptides like amyloid-β (Aβ). Aβ is the main component of brain and of the amyloid islets found in Alzheimer’s disease. Insulin is a peptide associated with diabetes. The gene encoding IDE is located on human chromosome 10q and is in a region linked to type 2 diabetes and Alzheimer’s disease. The deletion of IDE in mice leads to hyperinsulinemia, glucose intolerance and increases cerebral Aβ accumulation. Conversely, enhancement of IDE activity in neurons significantly reduces Aβ levels in the brain. IDE could help creating IDE-based therapies to control cerebral Aβ and blood sugar concentrations thanks to the understanding of its molecular mechanisms. IDE is an unusual enzyme because of its high affinity for substrates that are widely diverse in sequence and structure. IDE prefers to degrade <6 kDa bioactive peptides such as insulin, Aβ, glucagon, atrial natriuretic peptides or transforming growth factor α. Paradoxally, even though IDE targets a broad range of substrates, it shows a remarkable capacity to selectively cleave some peptides without degrading related family members.

How are the structure and the functions of IDE related?

Structure general points

IDE is a homodimeric protein. Each IDE monomer is composed of four 25-27 kDa structurally homologous αβ roll domains:

• : residues 43-285
• : residues 286-515
• : residues 542-768
• : residues 769-1,016

These domains share less than 25 % sequence similarity, and show similar secondary structures. The N-terminal domains 1 and 2 form a αβαβα sandwich (), as do domains 3 and 4 (). The size of each IDE-N and IDE-C is about 56 kDa. They are joined by a (residues 516-541) which interacts with both domains 2 and 3, but not with domains 1 and 4. There is an extensive interaction between IDE-N and IDE-C domains, that bury a large surface ( 11,200 Ȧ²), with good shape complementarity. This interaction between the two domains of IDE is mediated by charge complementarity and Van der Waals interactions. IDE-C is also involved in the oligomerization of IDE.

Catalytic chamber

IDE-N and IDE-C create substantial contact to form an enclosed catalytic chamber to encapsulate its substrates. This enclosed chamber is shaped like a triangular prism, with triangular base dimensions of 35x34x30 Ȧ and a height of 36 Ȧ. So the total volume of this prism is about 1.3x104 Ȧ³. All four domains contribute to surface the inner chamber. The surface provided by IDE-N is largely neutral or negatively charged, whereas that provided by IDE-C is mainly positively charged. The catalytic site of IDE is present within IDE-N, in the domain 1, but IDE-C is necessary for its catalytic activity. The contains a Zn²⁺ ion coordinated by two histidines (residues 108 and 112) and two glutamates (residues 111 and 189). The glutamate 111-residue is a base for the activation of a catalytic water molecule that mediates peptides hydrolysis. Moreover, these water molecules are involved in the coordination of the Zn²⁺ ion. At the catalytic site, these residues create a largely polar cavity with parts of hydrophobic and charged regions that interact with cleavage sites in many substrates. In the domain 2, there is an exosite that accommodates the N-terminus of substrates and is involved in the substrates binding.

Substrate binding

IDE can adopt two conformations, called “open” (IDE-o) and “closed” (IDE-c). In the open state, substrates can freely diffuse in and out of the catalytic chamber. In the close state, previously bound substrates are entrapped inside the catalytic chamber and repositioned to allow hydrolysis. The enclosed substrate-binding compartment of IDE prevents the entry and exit of substrates, so that substrates cannot be entrapped unless they were previously bound. IDE has to undergo a switch between these two states for catalysis. The enclosed substrate undergoes conformational changes to form β-sheets with two discrete regions of IDE for its degradation. IDE-N and IDE-C have extensive interactions that bury a large surface of 11,496 Ȧ² with good shape complementarity and numerous hydrogen bonds. In the absence of interactions with factors or proteins, the substrate-free IDE-c state is stable and the catalytic chamber of IDE is mostly closed. So we can consider that IDE-c is the resting and inactive state of IDE.

Han ethnicity-specific type 2 diabetic treatment from traditional Chinese medicine? ^[1]

Insulin-degrading enzyme (IDE) is a zinc-metalloendopeptidase found mostly in cytosol. Since overexpression of IDE increases the rate of extracellular insulin degradation, IDE is a potential target for controlling insulin degradation.

The (IDE protein colored in cyan and insulin colored in darkmagenta) is shown by the crystal structure 2g54. Leissring et al. (2010)^[2] also indicated a (colored in orange) and zinc ion in the catalytic site of IDE protein which interacted with their IDE inhibitors.

Virtual screening of the IDE protein (PDB ID: 2jg4) was conducted using the binding site defined by the catalytic site of IDE protein. In silico results indicate that traditional Chinese medicine compounds (colored in royalblue), (colored in blueviolet), and (colored in chocolate) had high binding affinity with IDE protein and formed hydrogen bonds with the key active residue, Glu111 (colored in magenta) and other residues in the IDE binding site (colored in green). As the top three TCM compounds had stable interactions with zinc cation and residues in the catalytic site of IDE, they may block binding of other substrates, such as insulin, to the catalytic site. This competitive binding may limit the degradation of insulin. The top TCM candidates, dihydrocaffeic acid, isopraeroside IV, and scopolin, may have potential to be lead compounds for controlling insulin degradation for type 2 diabetes mellitus.

3D structures of insulin-degrading enzyme

Insulin-degrading enzyme 3D structures