Test180919

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	==== Cis peptide bonds ====		==== Cis peptide bonds ====

-	The ω torsion angle can adopt a value close to 0° (cis-conformation), when a Pro residue is the following residue (Xaa-Pro peptide bond). In this situation a <scene name='~~User:Tilman_Schirmer~~/~~Sandbox_99~~/Pro_213_in_cis_conformation/4'>cis-Pro</scene> and a <scene name='User:Tilman_Schirmer/Sandbox_99/Pro_211_in_trans_conformation/2'>trans-Pro</scene> are similarily unfavorable, since there is a steric clash between Cα,i with Cα,i+1 or Cδ,i+1, respectively. Conversely, the carbonyl O of residue i is in tight juxtaposition with Cδ,i+1 or Cα,i+1 (note that latter tight contact occurs in any trans peptide bond).	+	The ω torsion angle can adopt a value close to 0° (cis-conformation), when a Pro residue is the following residue (Xaa-Pro peptide bond). In this situation a <scene name='82/824563/Pro_213_in_cis_conformation/1'>cis-Pro</scene> and a <scene name='User:Tilman_Schirmer/Sandbox_99/Pro_211_in_trans_conformation/2'>trans-Pro</scene> are similarily unfavorable, since there is a steric clash between Cα,i with Cα,i+1 or Cδ,i+1, respectively. Conversely, the carbonyl O of residue i is in tight juxtaposition with Cδ,i+1 or Cα,i+1 (note that latter tight contact occurs in any trans peptide bond).

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1 Peptide bond
2 Cis peptide bonds
3 Torsion angle φ restricted for Pro
4 References
5 Next Topic

Peptide bond

The (highlight in ) formation is a condensation reaction between the carboxyl group of the amino acid i and the amino group of the amino acid i+1.

The peptide bond is a resonance structure between two limiting states. Therefore the N-C bond has a partial double bond character and the atoms/groups C_α, HN, C, C=O are within one plane. Peptide bonds are usually in trans conformation (ω torsion angle= 180°). In cis-conformation, there would be steric hindrance between C_α,i and C_α,i+1.

Cis conformation has been observed almost exclusively for peptide bonds preceding a Pro residue (Xaa - Pro), see below.

is defined by the four atoms φ = C - N - C_α - C (in green).

is defined by the four atoms ψ = N - C_α - C - N (in green).

is defined by the four atoms ω = C_α - C - N - C_α (in green).

Cis peptide bonds

The ω torsion angle can adopt a value close to 0° (cis-conformation), when a Pro residue is the following residue (Xaa-Pro peptide bond). In this situation a and a are similarily unfavorable, since there is a steric clash between Cα,i with Cα,i+1 or Cδ,i+1, respectively. Conversely, the carbonyl O of residue i is in tight juxtaposition with Cδ,i+1 or Cα,i+1 (note that latter tight contact occurs in any trans peptide bond).

Torsion angle φ restricted for Pro

Due to the ring closure of the Pro side-chain the torsion around the N - C_α is considerably to a value between -50° to -90°.

References

Chakrabarti P, Pal D. The interrelationships of side-chain and main-chain conformations in proteins. Prog Biophys Mol Biol. 2001;76(1-2):1-102. PMID:11389934