2j44
From Proteopedia
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[[Image:2j44.gif|left|200px]] | [[Image:2j44.gif|left|200px]] | ||
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| - | | | + | {{STRUCTURE_2j44| PDB=2j44 | SCENE= }} |
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'''ALPHA-GLUCAN BINDING BY A STREPTOCOCCAL VIRULENCE FACTOR''' | '''ALPHA-GLUCAN BINDING BY A STREPTOCOCCAL VIRULENCE FACTOR''' | ||
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| + | ==Overview== | ||
| + | The ability of pathogenic bacteria to recognize host glycans is often essential to their virulence. Here we report structure-function studies of previously uncharacterized glycogen-binding modules in the surface-anchored pullulanases from Streptococcus pneumoniae (SpuA) and Streptococcus pyogenes (PulA). Multivalent binding to glycogen leads to a strong interaction with alveolar type II cells in mouse lung tissue. X-ray crystal structures of the binding modules reveal a novel fusion of tandem modules into single, bivalent functional domains. In addition to indicating a structural basis for multivalent attachment, the structure of the SpuA modules in complex with carbohydrate provides insight into the molecular basis for glycogen specificity. This report provides the first evidence that intracellular lung glycogen may be a novel target of pathogenic streptococci and thus provides a rationale for the identification of the streptococcal alpha-glucan-metabolizing machinery as virulence factors. | ||
==About this Structure== | ==About this Structure== | ||
2J44 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J44 OCA]. | 2J44 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J44 OCA]. | ||
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| + | ==Reference== | ||
| + | Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors., van Bueren AL, Higgins M, Wang D, Burke RD, Boraston AB, Nat Struct Mol Biol. 2007 Jan;14(1):76-84. Epub 2006 Dec 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17187076 17187076] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptococcus pneumoniae]] | [[Category: Streptococcus pneumoniae]] | ||
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[[Category: Higgins, M.]] | [[Category: Higgins, M.]] | ||
[[Category: Wang, D.]] | [[Category: Wang, D.]] | ||
| - | [[Category: | + | [[Category: Carbohydrate-binding module]] |
| - | [[Category: | + | [[Category: Glycogen binding]] |
| - | [[Category: | + | [[Category: Pullulanase]] |
| - | [[Category: | + | [[Category: Streptococcus pneumoniae]] |
| - | [[Category: | + | [[Category: Virulence]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:26:26 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:26, 30 April 2008
ALPHA-GLUCAN BINDING BY A STREPTOCOCCAL VIRULENCE FACTOR
Overview
The ability of pathogenic bacteria to recognize host glycans is often essential to their virulence. Here we report structure-function studies of previously uncharacterized glycogen-binding modules in the surface-anchored pullulanases from Streptococcus pneumoniae (SpuA) and Streptococcus pyogenes (PulA). Multivalent binding to glycogen leads to a strong interaction with alveolar type II cells in mouse lung tissue. X-ray crystal structures of the binding modules reveal a novel fusion of tandem modules into single, bivalent functional domains. In addition to indicating a structural basis for multivalent attachment, the structure of the SpuA modules in complex with carbohydrate provides insight into the molecular basis for glycogen specificity. This report provides the first evidence that intracellular lung glycogen may be a novel target of pathogenic streptococci and thus provides a rationale for the identification of the streptococcal alpha-glucan-metabolizing machinery as virulence factors.
About this Structure
2J44 is a Single protein structure of sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.
Reference
Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors., van Bueren AL, Higgins M, Wang D, Burke RD, Boraston AB, Nat Struct Mol Biol. 2007 Jan;14(1):76-84. Epub 2006 Dec 24. PMID:17187076 Page seeded by OCA on Wed Apr 30 13:26:26 2008
