2j8w
From Proteopedia
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'''THE CRYSTAL STRUCTURE OF CYTOCHROME C' FROM RUBRIVIVAX GELATINOSUS AT 1.3 A RESOLUTION AND PH 8.0''' | '''THE CRYSTAL STRUCTURE OF CYTOCHROME C' FROM RUBRIVIVAX GELATINOSUS AT 1.3 A RESOLUTION AND PH 8.0''' | ||
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| + | ==Overview== | ||
| + | The structure of the cytochrome c' from the purple non-sulfur phototrophic bacterium Rubrivivax gelatinosus was determined using two crystals grown independently at pH 6.3 and pH 8. The resolution attained for the two structures (1.29A and 1.50A for the crystals at high and low pH, respectively) is the highest to date for this class of proteins. The two structures were compared in detail in an attempt to investigate the influence of pH on the geometry of the haem and of the coordination environment of the Fe(III) ion. However, while the results suggest some small propensity for the movement of the metal atom out of the plane of the haem ring upon pH increase, the accuracy of the measurements at these two pH below the pK of the axial histidine is not sufficient to provide hard evidence of a shift in the iron position and associated changes. | ||
==About this Structure== | ==About this Structure== | ||
2J8W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rubrivivax_gelatinosus Rubrivivax gelatinosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J8W OCA]. | 2J8W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rubrivivax_gelatinosus Rubrivivax gelatinosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J8W OCA]. | ||
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| + | ==Reference== | ||
| + | High resolution crystal structure of Rubrivivax gelatinosus cytochrome c'., Benini S, Rypniewski WR, Wilson KS, Ciurli S, J Inorg Biochem. 2008 May;102(5-6):1322-8. Epub 2008 Jan 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18295896 18295896] | ||
[[Category: Rubrivivax gelatinosus]] | [[Category: Rubrivivax gelatinosus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Rubrivivax gelatinosus]] | [[Category: Rubrivivax gelatinosus]] | ||
[[Category: Transport]] | [[Category: Transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:27:01 2008'' |
Revision as of 10:27, 30 April 2008
THE CRYSTAL STRUCTURE OF CYTOCHROME C' FROM RUBRIVIVAX GELATINOSUS AT 1.3 A RESOLUTION AND PH 8.0
Overview
The structure of the cytochrome c' from the purple non-sulfur phototrophic bacterium Rubrivivax gelatinosus was determined using two crystals grown independently at pH 6.3 and pH 8. The resolution attained for the two structures (1.29A and 1.50A for the crystals at high and low pH, respectively) is the highest to date for this class of proteins. The two structures were compared in detail in an attempt to investigate the influence of pH on the geometry of the haem and of the coordination environment of the Fe(III) ion. However, while the results suggest some small propensity for the movement of the metal atom out of the plane of the haem ring upon pH increase, the accuracy of the measurements at these two pH below the pK of the axial histidine is not sufficient to provide hard evidence of a shift in the iron position and associated changes.
About this Structure
2J8W is a Single protein structure of sequence from Rubrivivax gelatinosus. Full crystallographic information is available from OCA.
Reference
High resolution crystal structure of Rubrivivax gelatinosus cytochrome c'., Benini S, Rypniewski WR, Wilson KS, Ciurli S, J Inorg Biochem. 2008 May;102(5-6):1322-8. Epub 2008 Jan 21. PMID:18295896 Page seeded by OCA on Wed Apr 30 13:27:01 2008
