6qnj
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 4.5 s timepoint== | |
| + | <StructureSection load='6qnj' size='340' side='right'caption='[[6qnj]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6qnj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QNJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QNJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qnj OCA], [http://pdbe.org/6qnj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qnj RCSB], [http://www.ebi.ac.uk/pdbsum/6qnj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qnj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU]] Involved in D-xylose catabolism. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase. | ||
| - | + | Liquid application method for time-resolved analyses by serial synchrotron crystallography.,Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D, Leimkohl JP, Schikora H, Schneider TR, Pearson AR, Tellkamp F, Miller RJD Nat Methods. 2019 Oct;16(10):979-982. doi: 10.1038/s41592-019-0553-1. Epub 2019, Sep 16. PMID:31527838<ref>PMID:31527838</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6qnj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Xylose isomerase]] | ||
[[Category: Mehrabi, P]] | [[Category: Mehrabi, P]] | ||
| - | [[Category: Miller, R | + | [[Category: Miller, R J.D]] |
| - | [[Category: Schulz, E | + | [[Category: Schulz, E C]] |
| + | [[Category: Complex]] | ||
| + | [[Category: Diffusion]] | ||
| + | [[Category: Dynamic]] | ||
| + | [[Category: Isomerase]] | ||
Revision as of 10:38, 2 October 2019
Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 4.5 s timepoint
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