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5ms1

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Revision as of 10:54, 2 October 2019

Cowpea mosaic virus top component (CPMV-T) - naturally occurring empty particles

Structural highlights

5ms1 is a 2 chain structure with sequence from Cpmv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[POL2_CPMVS] Movement protein: transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner.^[1] ^[2] ^[3] ^[4] The cleavable C-terminus of small coat protein seems to be involved in the packaging of the virion RNAs. Also seems to act as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.^[5] ^[6] ^[7] ^[8]

Publication Abstract from PubMed

Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.

The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy.,Hesketh EL, Meshcheriakova Y, Thompson RF, Lomonossoff GP, Ranson NA Sci Rep. 2017 Apr 3;7(1):539. doi: 10.1038/s41598-017-00533-w. PMID:28373698^[9]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Taylor KM, Spall VE, Butler PJ, Lomonossoff GP. The cleavable carboxyl-terminus of the small coat protein of cowpea mosaic virus is involved in RNA encapsidation. Virology. 1999 Mar 1;255(1):129-37. PMID:10049828
↑ Canizares MC, Taylor KM, Lomonossoff GP. Surface-exposed C-terminal amino acids of the small coat protein of Cowpea mosaic virus are required for suppression of silencing. J Gen Virol. 2004 Nov;85(Pt 11):3431-5. PMID:15483261 doi:http://dx.doi.org/85/11/3431
↑ Liu L, Grainger J, Canizares MC, Angell SM, Lomonossoff GP. Cowpea mosaic virus RNA-1 acts as an amplicon whose effects can be counteracted by a RNA-2-encoded suppressor of silencing. Virology. 2004 May 20;323(1):37-48. PMID:15165817 doi:http://dx.doi.org/10.1016/j.virol.2004.02.013
↑ Carvalho CM, Pouwels J, van Lent JW, Bisseling T, Goldbach RW, Wellink J. The movement protein of cowpea mosaic virus binds GTP and single-stranded nucleic acid in vitro. J Virol. 2004 Feb;78(3):1591-4. PMID:14722313
↑ Taylor KM, Spall VE, Butler PJ, Lomonossoff GP. The cleavable carboxyl-terminus of the small coat protein of cowpea mosaic virus is involved in RNA encapsidation. Virology. 1999 Mar 1;255(1):129-37. PMID:10049828
↑ Canizares MC, Taylor KM, Lomonossoff GP. Surface-exposed C-terminal amino acids of the small coat protein of Cowpea mosaic virus are required for suppression of silencing. J Gen Virol. 2004 Nov;85(Pt 11):3431-5. PMID:15483261 doi:http://dx.doi.org/85/11/3431
↑ Liu L, Grainger J, Canizares MC, Angell SM, Lomonossoff GP. Cowpea mosaic virus RNA-1 acts as an amplicon whose effects can be counteracted by a RNA-2-encoded suppressor of silencing. Virology. 2004 May 20;323(1):37-48. PMID:15165817 doi:http://dx.doi.org/10.1016/j.virol.2004.02.013
↑ Carvalho CM, Pouwels J, van Lent JW, Bisseling T, Goldbach RW, Wellink J. The movement protein of cowpea mosaic virus binds GTP and single-stranded nucleic acid in vitro. J Virol. 2004 Feb;78(3):1591-4. PMID:14722313
↑ Hesketh EL, Meshcheriakova Y, Thompson RF, Lomonossoff GP, Ranson NA. The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy. Sci Rep. 2017 Apr 3;7(1):539. doi: 10.1038/s41598-017-00533-w. PMID:28373698 doi:http://dx.doi.org/10.1038/s41598-017-00533-w

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ms1"

@@ Line 1: / Line 1: @@
 ==Cowpea mosaic virus top component (CPMV-T) - naturally occurring empty particles==
-<StructureSection load='5ms1' size='340' side='right' caption='[[5ms1]], [[Resolution|resolution]] 4.25&Aring;' scene=''>
+<StructureSection load='5ms1' size='340' side='right'caption='[[5ms1]], [[Resolution|resolution]] 4.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ms1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MS1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ms1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cpmv Cpmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MS1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MS1 FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ms1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ms1 OCA], [http://pdbe.org/5ms1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ms1 RCSB], [http://www.ebi.ac.uk/pdbsum/5ms1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ms1 ProSAT]</span></td></tr>
 </table>
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Cpmv]]
+[[Category: Large Structures]]
 [[Category: Hesketh, E L]]
 [[Category: Lomonossoff, G P]]

5ms1

From Proteopedia

Revision as of 10:54, 2 October 2019

Cowpea mosaic virus top component (CPMV-T) - naturally occurring empty particles

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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