This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2jzz
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2jzz.jpg|left|200px]] | [[Image:2jzz.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2jzz", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2jzz| PDB=2jzz | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''Solid-State NMR Structure of Microcrystalline Ubiquitin''' | '''Solid-State NMR Structure of Microcrystalline Ubiquitin''' | ||
| Line 28: | Line 25: | ||
[[Category: Manolikas, T.]] | [[Category: Manolikas, T.]] | ||
[[Category: Meier, B H.]] | [[Category: Meier, B H.]] | ||
| - | [[Category: | + | [[Category: Cytoplasm]] |
| - | [[Category: | + | [[Category: Protein microcrystal]] |
| - | [[Category: | + | [[Category: Signaling protein]] |
| - | [[Category: | + | [[Category: Ubl conjugation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:29:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:29, 30 April 2008
Solid-State NMR Structure of Microcrystalline Ubiquitin
Overview
Proton-driven 13C spin diffusion (PDSD) is a simple and robust two-dimensional NMR experiment. It leads to spectra with a high signal-to-noise ratio in which cross-peaks contain information about internuclear distances. We show that the total information content is sufficient to determine the atomic-resolution structure of a small protein from a single, uniformly 13C-, 15N-labeled microcrystalline sample. For the example of ubiquitin, the structure was determined by a manual procedure followed by an automatic optimization of the manual structure as well as by a fully automated structure determination approach. The relationship between internuclear distances and cross-peak intensities in the spectra is investigated.
About this Structure
2JZZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy., Manolikas T, Herrmann T, Meier BH, J Am Chem Soc. 2008 Mar 26;130(12):3959-66. Epub 2008 Mar 6. PMID:18321098 Page seeded by OCA on Wed Apr 30 13:29:01 2008
