2jzz
From Proteopedia
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'''Solid-State NMR Structure of Microcrystalline Ubiquitin''' | '''Solid-State NMR Structure of Microcrystalline Ubiquitin''' | ||
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[[Category: Manolikas, T.]] | [[Category: Manolikas, T.]] | ||
[[Category: Meier, B H.]] | [[Category: Meier, B H.]] | ||
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| - | [[Category: | + | [[Category: Signaling protein]] |
| - | [[Category: | + | [[Category: Ubl conjugation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:29:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:29, 30 April 2008
Solid-State NMR Structure of Microcrystalline Ubiquitin
Overview
Proton-driven 13C spin diffusion (PDSD) is a simple and robust two-dimensional NMR experiment. It leads to spectra with a high signal-to-noise ratio in which cross-peaks contain information about internuclear distances. We show that the total information content is sufficient to determine the atomic-resolution structure of a small protein from a single, uniformly 13C-, 15N-labeled microcrystalline sample. For the example of ubiquitin, the structure was determined by a manual procedure followed by an automatic optimization of the manual structure as well as by a fully automated structure determination approach. The relationship between internuclear distances and cross-peak intensities in the spectra is investigated.
About this Structure
2JZZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy., Manolikas T, Herrmann T, Meier BH, J Am Chem Soc. 2008 Mar 26;130(12):3959-66. Epub 2008 Mar 6. PMID:18321098 Page seeded by OCA on Wed Apr 30 13:29:01 2008
